Membrane-associated interleukin-1 promotes osteoclast-like cell formation in vitro

Bone and mineral Pub Date : 1994-04-01 DOI:10.1016/S0169-6009(08)80204-8

Tatsuji Nishihara , Tomihisa Takahashi , Yuichi Ishihara , Hidenobu Senpuku , Naoyuki Takahashi , Tatsuo Suda , Toshihiko Koga

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引用次数: 12

Abstract

The effect of paraformaldehyde-fixed murine macrophage P388D₁ cells on osteoclast-like cell formation was investigated in mouse marrow cultures. When mouse marrow cells were cocultured for 8 days with paraformaldehyde-fixed P388D₁ cells stimulated with lipopoly-saccaride (LPS), many tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells were formed. Non-stimulated, and paraformaldehyde-fixed P388D₁ cells did not induce the formation of TRACP-positive multinucleated cells. Salmon calcitonin and indomethacin strongly inhibited the paraformaldehyde-fixed, LPS-stimulated P388D₁ cells-induced TRACP-positive multinucleated cell formation. Monospecific anti-mouse recombinant IL-lα serum inhibited TRACP-positive multinucleated cell formation in the presence of paraformaldehyde-fixed P388D₁ cells stimulated with LPS. These results suggest that membrane-associated IL-1 on macrophages is responsible for the resorptive effect of paraformaldehyde-fixed P388D₁ cells stimulated with LPS.

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膜相关的白细胞介素-1促进体外破骨细胞样细胞的形成

在小鼠骨髓培养中研究多聚甲醛固定小鼠巨噬细胞P388D1细胞对破骨细胞样细胞形成的影响。将小鼠骨髓细胞与脂多糖(LPS)刺激的多聚甲醛固定的P388D1细胞共培养8天，形成了许多抗酒石酸酸性磷酸酶(TRACP)阳性的多核细胞。未刺激和多聚甲醛固定的P388D1细胞不诱导形成tracp阳性的多核细胞。鲑鱼降钙素和吲哚美辛强烈抑制多聚甲醛固定、lps刺激的P388D1细胞诱导的tracp阳性多核细胞形成。单特异性抗小鼠重组il -l - α血清抑制多聚甲醛固定P388D1细胞形成。这些结果表明，巨噬细胞上的膜相关IL-1负责多聚甲醛固定P388D1细胞在LPS刺激下的吸收作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Bone and mineral

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