[The membrane complex of Toxoplasma gondii: ultrastructural study of its constituents].

Abstract

Toxoplasma gondii membrane complex was fragmented by sonication and separated on a sucrose continuous gradient. Ultrastructural analysis of the 1,08 g/cm3 density fraction (that which corresponded with a maximal detection by the monoclonal antibody plasmalemma marker) showed an homogeneous population of small unimembrane vesicles. Ultrastructural analysis of the 1,13 g/cm3 density fraction (that with corresponded with a maximal detection by the monoclonal antibody inner complex marker) showed an heterogeneous population with unimembrane vesicles and trimembrane fragments.