Peptide synthesis catalyzed by the Glu/Asp-specific endopeptidase. Influence of the ester leaving group of the acyl donor on yield and catalytic efficiency.

J Bongers, W Liu, T Lambros, K Breddam, R M Campbell, A M Felix, E P Heimer
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Abstract

We recently described a two-step enzymatic semisynthesis of the superpotent analog of human growth hormone releasing factor, [desNH2Tyr1,D-Ala2,Ala15]-GRF(1-29)-NH2 (4), from the precursor, [Ala15,29]-GRF(4-29)-OH (1). C-Terminal amidation of 1 to form [Ala15]-GRF(4-29)-NH2 (2) was achieved by carboxypeptidase-Y-catalyzed exchange of Ala29-OH for Arg-NH2. The target analog 4 was then obtained by acylation of segment 2 with desNH2Tyr-D-Ala-Asp(OH)-OR (3) (R = CH3CH2- or 4-NO2C6H4CH2-) catalyzed by the V8 protease. In this paper we report on the use of the recently isolated Glu/Asp-specific endopeptidase (GSE) from Bacillus licheniformis, which is shown to be an efficient catalyst for the segment condensation of 2 and 3. GSE is more stable than the V8 protease under the conditions employed (20% DMF, pH 8.2, 37 degrees C). The extent of conversion of 2 into 4 is limited by proteolyses at Asp3-Ala4 and Asp25-Ile26. However, this proteolysis is virtually eliminated by use of the appropriate ester leaving group, R. A systematic study of the kinetics of the GSE-catalyzed segment condensations of 2 and a series of tripeptide esters, desNH2Tyr-D-Ala-Asp(OH)-OR (3) [R = CH3CH2- (3a), CH3- (3b), ClCH2CH2- (3c), C6H5CH2- (3d), 4-NO2C6H4CH2- (3e)] revealed that rate of aminolysis versus proteolysis, and hence the conversion of 2 into 4, increase with increasing specificity (Vmax/Km) of GSE for the tripeptide ester.(ABSTRACT TRUNCATED AT 250 WORDS)

由Glu/ asp特异性内肽酶催化的肽合成。酰基给体酯离去基对产率和催化效率的影响。
我们最近描述了两步酶促半合成人类生长激素释放因子的超强类似物[desNH2Tyr1,D-Ala2,Ala15]-GRF(1-29)-NH2(4),从前体[ala15,29]-GRF(4-29)-OH(1)。通过羧基肽酶y催化Ala29-OH交换Arg-NH2,实现了1的c端酰胺化,形成[Ala15]-GRF(4-29)-NH2(2)。然后在V8蛋白酶的催化下,用desNH2Tyr-D-Ala-Asp(OH)- or (3) (R = CH3CH2-或4- no2c6h4ch2 -)酰化第2段得到目标类似物4。本文报道了利用最近从地衣芽孢杆菌中分离到的Glu/ asp特异性内肽酶(GSE),该酶被证明是2和3片段缩合的有效催化剂。在所采用的条件下(20% DMF, pH 8.2, 37℃),GSE比V8蛋白酶更稳定。2转化为4的程度受到Asp3-Ala4和Asp25-Ile26蛋白水解的限制。然而,这种蛋白质水解实际上是通过使用适当的酯离开基团R来消除的。对GSE催化的2和一系列三肽酯,desnh2tyrr - d - ala - asp (OH)- or (3) [R = CH3CH2- (3a), CH3- (3b), ClCH2CH2- (3c), C6H5CH2- (3d), 4- no2c6h4ch2 - (3e)]的节段缩合动力学的系统研究表明,随着GSE对三肽酯的特异性(Vmax/Km)的增加,氨基水解速率和蛋白质水解速率以及2转化为4的速度都在增加。(摘要删节250字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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