Thermostability of bacterial luciferase expressed in different microbes.

B M Mackey, D Cross, S F Park
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引用次数: 13

Abstract

Bacterial luciferase was used to investigate the relationship between the thermostability of a cytoplasmic reporter molecule and cellular heat resistance. The luciferase activity of Vibrio fischeri was expressed in strains of Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Brochothrix thermosphacta following transformation with plasmid pSP13 carrying the luxAB genes. The thermostability of intracellular luciferase varied depending on the organism in which it was expressed, but was not related to the cellular heat resistance of the different organisms. Addition of xylitol to the heating medium protected against loss of viability and inactivation of intracellular luciferase. Glycerol also protected against loss of viability but was less effective at preventing thermal denaturation of luciferase.

细菌荧光素酶在不同微生物中表达的热稳定性。
利用细菌荧光素酶研究细胞质报告分子的热稳定性与细胞耐热性之间的关系。用携带luxAB基因的质粒pSP13转染大肠杆菌、鼠伤寒沙门菌、单核增生李斯特菌和热嗜菌后,fischeri弧菌荧光素酶活性得到表达。细胞内荧光素酶的热稳定性取决于其表达的生物体,但与不同生物体的细胞耐热性无关。在加热介质中加入木糖醇可以防止细胞内荧光素酶丧失活力和失活。甘油也可以防止活力丧失,但在防止荧光素酶热变性方面效果较差。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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