Study of gene transfer in vitro and in the digestive tract of gnotobiotic mice from Lactococcus lactis strains to various strains belonging to human intestinal flora.

M Gruzza, M Fons, M F Ouriet, Y Duval-Iflah, R Ducluzeau
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Abstract

The use of genetically modified organisms (GMO) in dairy products requires evaluation of the DNA transfer capacity from such organisms among the human intestinal microflora. Thus, both in vitro and in vivo [in the digestive tract (DT) of mice] transfer from Lactococcus lactis donor strains of the conjugative plasmid pIL205 (CmR) and the non-conjugative plasmid pIL253 (EmR) to: (1) recipient strains isolated from human faecal flora Bacteroides sp., Bifidobacterium sp., Peptostreptococcus sp. (strictly anaerobic bacterial strains) and Enterococcus faecalis, (2) a whole human faecal flora, was studied. In both cases, no gene transfer was observed to strictly anaerobic bacterial strains. DNA transfer was only observed to the E. faecalis strain: in vivo CmR E. faecalis transconjugants were isolated from sequentially multi-associated mice and when the recipient strains associated with the mice, they were a defined mixture of Bacteroides sp., Bifidobacterium sp., Peptostreptococcus sp. and E. faecalis strains. When mice were associated with the whole human faecal flora, the plasmid pIL205 was transferred into some facultative anaerobic streptococci. It was also shown that DNA transfer occurred even when the lactococcal donor strain was transient in the DT of the gnotobiotic host animals.

乳酸乳球菌基因在体外和无菌小鼠消化道内向人类肠道菌群中不同菌株转移的研究。
在乳制品中使用转基因生物(GMO)需要评估这些生物在人类肠道微生物群中的DNA转移能力。因此,在体外和体内[在小鼠消化道(DT)中]研究了乳酸乳球菌的共轭质粒pIL205 (CmR)和非共轭质粒pIL253 (EmR)向:(1)从人类粪便菌群拟杆菌、双歧杆菌、Peptostreptococcus sp.(严格的厌氧菌株)和粪肠球菌(Enterococcus faecalis)中分离的受体菌株的转移,(2)整个人类粪便菌群。在这两种情况下,没有观察到基因转移到严格的厌氧细菌菌株。仅观察到粪肠杆菌菌株的DNA转移:在体内CmR中,从顺序多关联小鼠中分离出粪肠杆菌转偶联体,当与小鼠相关的受体菌株时,它们是明确的拟杆菌属、双歧杆菌属、胃链球菌属和粪肠杆菌菌株的混合物。当小鼠与整个人类粪便菌群相关联时,将质粒pIL205转移到一些兼性厌氧链球菌中。研究还表明,即使乳球菌供体菌株在非生物宿主动物的DT中是短暂的,DNA转移也会发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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