Serum enzyme activities in full-term asphyxiated and healthy newborns: enzyme kinetics during the first 144 hours of life.

Enzyme & protein Pub Date : 1993-01-01 DOI:10.1159/000468672
G M Lackmann, U Töllner, R Mader
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引用次数: 48

Abstract

Little is known about the kinetics of most serum enzymes during the first hours of life, and even less about the effect on such enzyme activities of perinatal hypoxia-ischaemia. It was the aim of the present study to evaluate the serum kinetics of seven differently located cell enzymes in healthy and asphyxiated newborns during the 1st week of life. The serum activities of cytoplasmic and mitochondrial [aspartate aminotransferase (ASAT), creatine kinase (CK), glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH), and hydroxybutyrate dehydrogenase (HBDH)] and membrane-bound (gamma-glutamyl-transferase and leucine arylaminidase) enzymes were prospectively measured in full-term asphyxiated (n = 49) and healthy (n = 87) newborns during the first 144 h of life. The blood samples were taken serially at five fixed times: 0 (cord), 12, 24, 72, and 144 h postpartum. The asphyxiated newborns had significantly increased serum activities of ASAT, LDH, and HBDH up to 72 h postpartum, whereas healthy newborns showed higher CK and GLDH activities. Only the activities of ASAT, LDH, and HBDH seemed to depend on the oxygen supply of the fetus or newborn. If other causes of increased serum enzyme activities, e.g. liver diseases, haemolytic disorders, tumours, or inborn errors of metabolism, are excluded, elevated serum activities of ASAT, LDH, and HBDH should draw one's attention to a perinatal hypoxic-ischaemic insult of the newborn.

足月窒息和健康新生儿血清酶活性:生命最初144小时的酶动力学
在生命最初的几个小时内,大多数血清酶的动力学知之甚少,而围产期缺氧缺血对这些酶活性的影响就更少了。本研究的目的是评估健康和窒息新生儿在生命第一周内七种不同位置细胞酶的血清动力学。对49例足月窒息新生儿(n = 49)和87例健康新生儿(n = 87)在出生后144小时内细胞质和线粒体[天冬氨酸转氨酶(ASAT)、肌酸激酶(CK)、谷氨酸脱氢酶(GLDH)、乳酸脱氢酶(LDH)和羟丁酸脱氢酶(HBDH)]及膜结合(γ -谷氨酰基转移酶和赖氨酸氨基酶)酶的血清活性进行了前瞻性测定。分别于产后0(脐带)、12、24、72、144 h五个固定时间连续采血。窒息新生儿的血清ASAT、LDH和HBDH活性在产后72 h显著升高,而健康新生儿的CK和GLDH活性较高。只有ASAT、LDH和HBDH的活性似乎依赖于胎儿或新生儿的氧供应。如果排除其他导致血清酶活性升高的原因,如肝脏疾病、溶血性疾病、肿瘤或先天性代谢错误,则血清ASAT、LDH和HBDH活性升高应引起人们对围产期新生儿缺氧缺血性损伤的注意。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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