In situ flat embedding of monolayers and cell relocation in the acrylic resin LR white for comparative light and electron microscopy studies.

The Histochemical Journal Pub Date : 1994-12-01
M Steiner, C Schöfer, W Mosgoeller
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Abstract

A simple and reliable method has been developed for the in situ LR White embedding of cell monolayers grown on glass cover-slips. Combined with cytochemical or immunological procedures, this technique allows light and/or electron microscopy investigations of a large number of cells in the same horizontal plane within a relatively short period of time. It can be applied to cells grown on microgrid finder cover-slips which allows a distinct site of even an individual cell of a monolayer to be studied at first at the light microscope level and subsequently at the electron microscope level. Hence, it is also suitable for controlling manipulation of single cells, followed by their serial sectioning after relocation in the electron microscope.

在丙烯酸树脂LR白中原位平埋单层和细胞重新定位的比较光学和电子显微镜研究。
建立了一种简单、可靠的细胞单层原位LR白包埋法。结合细胞化学或免疫学程序,该技术允许在相对较短的时间内对同一水平面上的大量细胞进行光学和/或电子显微镜调查。它可以应用于生长在微网格探测器覆盖卡瓦上的细胞,它允许在光学显微镜水平和随后的电子显微镜水平上首先研究单个单层细胞的不同位置。因此,它也适用于单细胞的控制操作,然后在电子显微镜下重新定位后进行连续切片。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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