The effect of reserpine treatment in vivo upon L-dopa and amphetamine evoked dopamine and DOPAC efflux in vitro from the corpus striatum of male rats.

D E Dluzen, B Liu
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引用次数: 4

Abstract

In the present experiment we tested the effects of L-DOPA and amphetamine upon dopamine and DOPAC efflux in vitro from superfused corpus striatal tissue fragments of male rats who had been pretreated with reserpine. Male rats were treated with reserpine (5 mg/kg) or its vehicle at 24 hours prior to sacrifice and superfusion of the corpus striatum. Two different modes of L-DOPA (5 microM) and amphetamine (10 microM) stimulation, a brief 10-minute and a continuous 60-minute infusion, were tested for their ability to evoke striatal dopamine and DOPAC efflux. Depletion of monoamine storage capacity as achieved with reserpine significantly reduced the amount of basal dopamine and DOPAC released from superfused striatal tissue fragments of male rats. Although basal release rates were significantly reduced, the amount of dopamine and DOPAC released in response to in vitro L-DOPA infusions (10 or 60 minute infusions) was equivalent between reserpine and vehicle treated animals. In contrast, amphetamine stimulated DA release was significantly reduced in male rats treated with reserpine. For both L-DOPA and amphetamine, significantly greater amounts of dopamine were obtained with the 60- versus 10-minute infusion modes. These results demonstrate that the capacity for L-DOPA, but not amphetamine, to evoke dopamine efflux is unaltered under conditions when monoamine storage ability is diminished.

利血平对体内左旋多巴和安非他明的影响诱导雄性大鼠纹状体多巴胺和多巴胺外排。
本实验研究了左旋多巴和安非他明对利血平预处理的雄性大鼠纹状体多余组织片段体外多巴胺和多巴胺外排的影响。雄性大鼠在纹状体灌注前24小时给予利血平(5 mg/kg)或其载药。两种不同模式的左旋多巴(5微米)和安非他明(10微米)刺激,短暂的10分钟和连续的60分钟输注,测试了他们唤起纹状体多巴胺和DOPAC外排的能力。利血平减少了雄性大鼠的单胺储存能力,显著降低了过量纹状体组织碎片释放的基础多巴胺和DOPAC的量。虽然基础释放率显著降低,但体外左旋多巴输注(输注10或60分钟)释放的多巴胺和DOPAC的量在利血平和载药处理的动物之间是相同的。相反,安非他明刺激的DA释放在利血平治疗的雄性大鼠中显著减少。对于左旋多巴和安非他明,60分钟输注模式比10分钟输注模式获得的多巴胺量明显更多。这些结果表明,在单胺储存能力减弱的情况下,左旋多巴(而非安非他明)引起多巴胺外排的能力没有改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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