Cell proliferation in prostatic carcinoma: comparative analysis of Ki-67, MIB-1 and PCNA.

The Histochemical Journal Pub Date : 1995-03-01
P J Hepburn, E Glynne-Jones, L Goddard, J M Gee, M E Harper
{"title":"Cell proliferation in prostatic carcinoma: comparative analysis of Ki-67, MIB-1 and PCNA.","authors":"P J Hepburn,&nbsp;E Glynne-Jones,&nbsp;L Goddard,&nbsp;J M Gee,&nbsp;M E Harper","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Antibodies to assess the proliferative index of tumours are being increasingly employed together with established markers for prognostic evaluation. This study set out to compare three cell proliferation markers, Ki-67, MIB-1 and PCNA, utilizing a semi-quantitative method of assessment, in 20 human prostatic carcinomas. The streptavidin-biotin immunostaining system was used for the monoclonal antibodies MIB-1 and PCNA and an indirect immunoperoxidase assay for the monoclonal antibody Ki-67. Significant correlations were found between the expression of Ki-67 in frozen tissues and MIB-1 in formal saline-fixed wax-embedded tissues (p = 0.0003); between Ki-67 and PCNA expression in Bouin's-fixed tissues (p < or = 0.0001); and MIB-1 (formalin-saline-fixed tissues) and PCNA (Bouin's-fixed tissues) (p < or = 0.0001). A more intense nuclear staining pattern with less heterogeneity was observed for MIB-1 compared with PCNA, suggesting the antibody of choice, on formal saline-fixed tissues, is MIB-1, which closely correlated with Ki-67, a marker we have previously shown to be of prognostic value in prostatic carcinoma.</p>","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":"27 3","pages":"196-203"},"PeriodicalIF":0.0000,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Histochemical Journal","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Antibodies to assess the proliferative index of tumours are being increasingly employed together with established markers for prognostic evaluation. This study set out to compare three cell proliferation markers, Ki-67, MIB-1 and PCNA, utilizing a semi-quantitative method of assessment, in 20 human prostatic carcinomas. The streptavidin-biotin immunostaining system was used for the monoclonal antibodies MIB-1 and PCNA and an indirect immunoperoxidase assay for the monoclonal antibody Ki-67. Significant correlations were found between the expression of Ki-67 in frozen tissues and MIB-1 in formal saline-fixed wax-embedded tissues (p = 0.0003); between Ki-67 and PCNA expression in Bouin's-fixed tissues (p < or = 0.0001); and MIB-1 (formalin-saline-fixed tissues) and PCNA (Bouin's-fixed tissues) (p < or = 0.0001). A more intense nuclear staining pattern with less heterogeneity was observed for MIB-1 compared with PCNA, suggesting the antibody of choice, on formal saline-fixed tissues, is MIB-1, which closely correlated with Ki-67, a marker we have previously shown to be of prognostic value in prostatic carcinoma.

前列腺癌细胞增殖:Ki-67、mb -1和PCNA的比较分析。
用于评估肿瘤增殖指数的抗体正越来越多地与已建立的预后评估标志物一起使用。本研究利用半定量评估方法比较了20例人前列腺癌中Ki-67、mb -1和PCNA三种细胞增殖标志物。单克隆抗体mb -1和PCNA采用链亲和素-生物素免疫染色系统,单克隆抗体Ki-67采用间接免疫过氧化物酶法。Ki-67在冷冻组织中的表达与mb -1在盐水固定蜡包埋组织中的表达呈显著相关(p = 0.0003);Bouin固定组织中Ki-67与PCNA表达差异(p < or = 0.0001);MIB-1(福尔马林-盐固定组织)和PCNA (Bouin -s固定组织)(p <或= 0.0001)。与PCNA相比,MIB-1的核染色模式更强烈,异质性更小,这表明在正式的盐固定组织中,选择的抗体是MIB-1,它与Ki-67密切相关,Ki-67是我们之前显示的前列腺癌预后价值的标记物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信