Immunogold labelling indicates high catalase concentrations in amorphous and crystalline inclusions of sunflower (Helianthus annuus L.) peroxisomes.

The Histochemical Journal Pub Date : 1995-03-01
K B Tenberge, R Eising
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Abstract

Immunogold labelling and electron microscopy were used to investigate whether catalase was present in peroxisomal inclusions, the composition of which has not yet been determined in plant cells. In the mesophyll cells of sunflower (Helianthus annuus L.) cotyledons, the catalase gold label was confined to peroxisomes. At day 2 of postgerminative growth in darkness, peroxisomes were free of inclusions, and the matrix was homogeneously labelled with gold particles. Thereafter, amorphous inclusions appeared, but by day 5 of growth, conspicuous crystalline inclusions (cores) were the predominant type. This developmental change, first observed in cotyledons grown in continuous light between day 2.5 and 5, also took place in cotyledons kept in permanent darkness. Both amorphous and crystalline inclusions showed a much higher immunogold label than did the peroxisomal matrix, indicating that catalase was a component of both types of peroxisomal inclusions. In contrast to catalase, the immunogold label of glycolate oxidase was almost completely absent from cores and was confined to the peroxisomal matrix. Together with reports on the absence of other enzymes from peroxisomal inclusions in sunflower and other species (Vaughn, 1989) our results suggest that catalase is a major constituent of amorphous and crystalline peroxisomal inclusions in plants.

免疫金标记表明,在向日葵(Helianthus annuus L.)过氧化物酶体的无定形和结晶包涵体中有高浓度的过氧化氢酶。
利用免疫金标记和电子显微镜研究过氧化氢酶是否存在于植物细胞中尚未确定其组成的过氧化物酶体内含物中。在向日葵子叶叶肉细胞中,过氧化氢酶金标记仅限于过氧化物酶体。在黑暗中萌发后生长的第2天,过氧化物酶体没有夹杂物,基质被金颗粒均匀标记。之后,出现非晶态夹杂物,但在生长第5天,明显的结晶夹杂物(岩心)成为主要类型。这种发育变化首先在连续光照2.5 - 5天的子叶中观察到,也发生在长期黑暗中的子叶中。无定形和结晶包涵体的免疫金标记都比过氧化物酶体基质高得多,这表明过氧化氢酶是两种过氧化物酶体包涵体的组成部分。与过氧化氢酶相比,乙醇酸氧化酶的免疫金标记几乎完全不存在于核中,并且局限于过氧化物酶体基质。加上关于向日葵和其他物种的过氧化物酶体内含物中缺乏其他酶的报道(Vaughn, 1989),我们的研究结果表明,过氧化氢酶是植物中无定形和结晶过氧化物酶体内含物的主要成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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