Detection of a glycosphingolipid antigen in bladder cancer cells with monoclonal antibody MRG-1.

The Histochemical Journal Pub Date : 1995-03-01
S Kurimoto, N Moriyama, K Takata, S Nozawa, Y Aso, H Hirano
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Abstract

The monoclonal antibody MRG-1 has been evaluated for the immunohistochemical detection of the type 3 chain of blood group A in human normal bladder epithelium and bladder tumours. Light microscope examination of paraffin sections demonstrated that this antigen was present in normal epithelium and superficial bladder tumour in patients with blood group A or AB, but was absent in the invasive type of bladder tumour. In normal epithelium, the plasma membrane was positive for this antigen, and the cytoplasm was diffusely stained. In superficial transitional cell carcinoma, the plasma membrane was negative, whereas the cytoplasm was intensely stained in the perinuclear region. This pattern was different from that observed for type 1 and 2 group A antigen, which was recognized mainly at the plasma membrane. However, in superficial transitional cell carcinoma, the staining was also seen on the plasma membrane. The pattern of the localization of this antigen in this carcinoma was influenced by the treatment of organic solvents. Electron microscopical observations confirmed that this antigen was localized on the plasma membrane and also in the Golgi apparatus of the superficial tumour. These results proved that the type 3 chain of blood group A is present in human bladder epithelium and low grade tumours in correspondence with the blood type, but disappears in tumours with high malignant potential. However, its expression is independent of the expressions of the other subtypes which have been studied. Furthermore, the changes in the staining pattern caused by pretreatment with organic solvents suggested possible differences in the microenvironment of the glycolipids containing this type of sugar chain.

单克隆抗体MRG-1检测膀胱癌细胞中鞘糖脂抗原。
单克隆抗体MRG-1已被评价用于人正常膀胱上皮和膀胱肿瘤中A血型3型链的免疫组化检测。石蜡切片光镜检查显示,该抗原存在于A或AB血型患者的正常上皮和浅表性膀胱肿瘤中,但在浸润型膀胱肿瘤中不存在。在正常上皮中,质膜对该抗原呈阳性,细胞质弥漫性染色。在浅表移行细胞癌中,质膜呈阴性,而胞质在核周区呈强烈染色。这种模式与1型和2型A组抗原不同,它们主要在质膜上被识别。而浅表移行细胞癌,质膜上也可见染色。这种抗原在这种癌中的定位模式受到有机溶剂处理的影响。电镜观察证实该抗原定位于肿瘤表面的质膜和高尔基体。这些结果证明,A血型3型链在人膀胱上皮和低级别肿瘤中存在与血型相符的存在,但在高恶性潜能肿瘤中消失。然而,它的表达独立于已研究的其他亚型的表达。此外,有机溶剂预处理引起的染色模式的变化表明含有这种糖链的糖脂的微环境可能存在差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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