A sensitive enzyme-linked immunosorbent assay of serum ornithine carbamoyltransferase.

Enzyme & protein Pub Date : 1994-01-01 DOI:10.1159/000474964
Y Watanabe, S Mori, M Ozaki, S Fujiyama, T Sato, M Mori
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引用次数: 5

Abstract

Ornithine carbamoyltransferase (OCT), a urea cycle enzyme, is located almost exclusively in liver mitochondria. We designed a sensitive enzyme-linked immunosorbent assay (ELISA) of serum OCT protein using an antibody against purified bovine enzyme. OCT protein measured using this method showed a good correlation with OCT activity (r = 0.961), and was much higher in patients with liver diseases than in the controls. Measurements of serum OCT protein in 442 healthy blood donors gave the upper limit of normal range of 23 ng Eq/ml (equivalent to the bovine enzyme) for males, 8 ng Eq/ml for females, and 16 ng Eq/ml for males plus females. The values differ significantly between the sexes and depending on ages. This ELISA system is expected to be used as a pertinent liver function test.

血清鸟氨酸氨基甲酰转移酶的灵敏酶联免疫吸附测定。
鸟氨酸氨基甲酰转移酶(OCT)是一种尿素循环酶,几乎只存在于肝脏线粒体中。我们设计了一种灵敏的血清OCT蛋白酶联免疫吸附试验(ELISA),使用一种针对纯化牛酶的抗体。用该方法测得的OCT蛋白与OCT活性具有良好的相关性(r = 0.961),且肝脏疾病患者OCT蛋白的相关性远高于对照组。对442名健康献血者的血清OCT蛋白测量显示,正常范围的上限为男性23 ng Eq/ml(相当于牛酶),女性8 ng Eq/ml,男性加女性16 ng Eq/ml。这些值在性别和年龄之间差别很大。该ELISA系统有望用于相关的肝功能检测。
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