Effects of estrogen on apoptotic pathways in human breast cancer cell line MCF-7.

IF 12.5 1区医学 Q1 ONCOLOGY

Cancer research Pub Date : 1995-06-15

T T Wang, J M Phang

引用次数: 0

Abstract

We utilized a reverse transcription-PCR method to examine the effect of estrogen on the expression of mRNA for Bcl-2 and Bax, two modulatory proteins in the apoptotic pathway, in human breast cancer cell line MCF-7. We found that the bcl-2 mRNA levels in the cells exposed to 17 beta-estradiol were higher than those of control cells. Although the relative bax mRNA levels remained unchanged, the changes in bcl-2 mRNA level occurred in a time- and concentration-dependent fashion. In addition, pretreatment with 17 beta-estradiol protected MCF-7 cells from apoptosis. Our study provides evidence that responses of breast epithelial cells toward a steroid sex hormone involve regulation of the apoptotic pathway.

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雌激素对人乳腺癌细胞系MCF-7凋亡通路的影响。

我们利用逆转录pcr方法检测了雌激素对人乳腺癌MCF-7细胞凋亡通路中两种调节蛋白Bcl-2和Bax mRNA表达的影响。我们发现，暴露于17 -雌二醇的细胞中bcl-2 mRNA水平高于对照细胞。虽然相对bax mRNA水平保持不变，但bcl-2 mRNA水平的变化呈时间和浓度依赖性。此外，17 -雌二醇预处理可保护MCF-7细胞免于凋亡。我们的研究提供了证据，证明乳腺上皮细胞对类固醇性激素的反应涉及凋亡途径的调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer research 医学-肿瘤学

CiteScore

16.10

自引率

0.90%

发文量

7677

审稿时长

2.5 months

期刊介绍： Cancer Research, published by the American Association for Cancer Research (AACR), is a journal that focuses on impactful original studies, reviews, and opinion pieces relevant to the broad cancer research community. Manuscripts that present conceptual or technological advances leading to insights into cancer biology are particularly sought after. The journal also places emphasis on convergence science, which involves bridging multiple distinct areas of cancer research. With primary subsections including Cancer Biology, Cancer Immunology, Cancer Metabolism and Molecular Mechanisms, Translational Cancer Biology, Cancer Landscapes, and Convergence Science, Cancer Research has a comprehensive scope. It is published twice a month and has one volume per year, with a print ISSN of 0008-5472 and an online ISSN of 1538-7445. Cancer Research is abstracted and/or indexed in various databases and platforms, including BIOSIS Previews (R) Database, MEDLINE, Current Contents/Life Sciences, Current Contents/Clinical Medicine, Science Citation Index, Scopus, and Web of Science.