Interleukin-15 and the growth of tumor derived activated T-cells.

W M Lewko, T L Smith, D J Bowman, R W Good, R K Oldham
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引用次数: 36

Abstract

Interleukin-15 was tested to determine whether this recently discovered cytokine was capable of stimulating the growth of tumor derived activated T cells in culture (TDAC, also referred to as tumor infiltrating lymphocytes). When established cultures of IL-2 induced, IL-2 dependent TDAC were tested, IL-15 stimulated growth in a dose dependent manner, alone or in the presence of IL-2. One established TDAC was cultured with IL-15 alone for 18 passages over a 10 week period. Comparing IL-2 and IL-15 treated cultures, growth rate with IL-15 was slower. IL-15 doubled the secreted interferon alpha and granulocyte-macrophage colony stimulating factor. IL-15 and IL-2 were compared in primary TDAC cultures. IL-15 induced TDAC outgrowth in 3 of 6 cultures. IL-2 induced outgrowth in all 6. Tumor cells were eliminated as TDAC grew out in both IL-2 and IL-15 treated cultures. These results suggested that IL-15 like IL-2, is capable of stimulating the growth of TDAC with antitumor activity, but with certain distinct effects which may be of interest therapeutically.

白细胞介素-15与肿瘤源性活化t细胞的生长。
研究人员对白细胞介素-15进行了测试,以确定这种最近发现的细胞因子是否能够刺激培养的肿瘤源性活化T细胞(TDAC,也称为肿瘤浸润淋巴细胞)的生长。当对IL-2诱导的、依赖IL-2的TDAC进行培养时,IL-15以剂量依赖的方式刺激生长,单独或在IL-2存在的情况下。一个已建立的TDAC与IL-15单独培养18代,持续10周。与IL-2和IL-15处理的培养物相比,IL-15的生长速度较慢。IL-15使分泌的α干扰素和粒细胞-巨噬细胞集落刺激因子增加一倍。在原代TDAC培养中比较IL-15和IL-2。IL-15在6个培养物中的3个中诱导了TDAC的生长。IL-2诱导了所有6例细胞的生长。在IL-2和IL-15处理的培养物中,肿瘤细胞随着TDAC的生长而消失。这些结果表明,IL-15与IL-2一样,能够刺激TDAC的生长,具有抗肿瘤活性,但具有一定的独特作用,可能具有治疗意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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