{"title":"A practical method for fission yeast transformation by electroporation.","authors":"J Ishiguro, W Kobayashi","doi":"10.1266/jjg.70.1","DOIUrl":null,"url":null,"abstract":"<p><p>High-voltage shock within a very short duration under the proper conditions causes cells to incorporate exogenous macromolecules. This technique, electroporation, has been widely used in recent years to transform many organisms. We determined optimum conditions for fission yeast transformation using this method. Of nineteen combinations of electric field strength and pulse time examined, 1.75 kV/0.2 cm, 4 msec pulse was found to provide approximately 4.0 x 10(5) transformants per micrograms of DNA. Other factors responsible for the transformation efficiency in fission yeast are also discussed.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 1","pages":"1-6"},"PeriodicalIF":0.0000,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.1","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Idengaku zasshi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1266/jjg.70.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
High-voltage shock within a very short duration under the proper conditions causes cells to incorporate exogenous macromolecules. This technique, electroporation, has been widely used in recent years to transform many organisms. We determined optimum conditions for fission yeast transformation using this method. Of nineteen combinations of electric field strength and pulse time examined, 1.75 kV/0.2 cm, 4 msec pulse was found to provide approximately 4.0 x 10(5) transformants per micrograms of DNA. Other factors responsible for the transformation efficiency in fission yeast are also discussed.
在适当条件下，极短时间内的高压冲击可使细胞吸收外源性大分子。近年来，电穿孔技术已被广泛应用于许多生物的转化。我们用这种方法确定了裂变酵母转化的最佳条件。在19种电场强度和脉冲时间的组合中，发现1.75 kV/0.2 cm, 4 msec脉冲每微克DNA提供大约4.0 x 10(5)个变子。对影响裂变酵母转化效率的其他因素也进行了讨论。
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