Antibody production of a human EBV-transformed B cell line and its heterohybridoma and trioma cell line descendants in different culture systems.

Human antibodies and hybridomas Pub Date : 1994-01-01
B Gustafsson, J Hinkula
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Abstract

Cells of an EBV-transformed human lymphoblastoid B cell line, producing antibodies directed against tetanus toxin, were fused with mouse myeloma cells (SP2/0) and with mouse-human heteromyeloma cells (SPAM-8) resulting in the formation of heterohybridoma and trioma cells, respectively. Antibody production of the three cell lines were studied under different culture conditions. All three cell lines produced antibodies in concentrations ranging from 2.6 to 6.4 micrograms ml-1 in spent medium from stationary flask cultures. Dialysis cultures of trioma and heterohybridoma cells resulted in concentrations of 36 and 20 micrograms ml-1, respectively, whereas no significant increase was obtained with the EBV-transformed cells. Trioma cells, cultured in a hollow fiber cartridge bioreactor produced antibodies in concentrations of average of 303 micrograms ml-1, whereas the EBV-transformed cells did not adapt to this system. Furthermore, trioma and heterohybridoma cells injected into the intraperitoneal cavity of SCID-mice, produced antibodies in ascites fluid in concentrations of 500 and 640 micrograms ml-1 respectively.

人ebv转化B细胞系及其异杂交瘤和三瘤细胞系后代在不同培养体系中的抗体产生
将ebv转化的人淋巴母细胞样B细胞系细胞与小鼠骨髓瘤细胞(SP2/0)和小鼠-人异骨髓瘤细胞(SPAM-8)融合,分别形成异杂交瘤细胞和三瘤细胞。研究了三种细胞系在不同培养条件下产生抗体的情况。所有三种细胞系在固定烧瓶培养的废培养基中产生的抗体浓度范围为2.6至6.4微克ml-1。三瘤细胞和杂交瘤细胞的透析培养浓度分别为36和20微克ml-1,而ebv转化的细胞没有显著增加。在中空纤维筒式生物反应器中培养的三角瘤细胞产生的抗体平均浓度为303微克ml-1,而ebv转化的细胞不适应该系统。此外,将三瘤和异杂交瘤细胞注射到scid小鼠腹腔内,在腹水中分别产生浓度为500和640微克ml-1的抗体。
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