B-cells in thymic epithelial tumours. An immunohistochemical analysis of intra- and extraepithelial B-cell compartments.

F Fend, T Kirchner, A Marx, H K Müller-Hermelink
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Abstract

A total of 26 thymomas and thymic carcinomas were studied by immunohistochemistry to determine the presence and distribution of intratumoural B-cells. Double staining experiments revealed two distinct B-cell populations in the thymic epithelial tumours. One was found within the perivascular space (PVS), which is separated from the neoplastic epithelium by a basement membrane. In all tumours the PVS contained lymphocytes with the immunophenotype of peripheral B-cells. Large numbers of B-cells with germinal centre formation were found almost exclusively in myasthenia gravis (MG)-associated tumours, mainly in cortical thymomas and well differentiated thymic carcinomas. A second population of B-cells was located in the neoplastic epithelial meshwork, mostly in areas of organoid medullary differentiation characterized by epidermoid cells or Hassall's corpuscules. This population frequently comprised large, CD23+ cells with dendritic features resembling the special type of intramedullary B-cells of the normal human thymus. In contrast, B-cells were uncommon in areas of mixed thymoma showing spindle celled medullary differentiation, and were almost completely absent from tumour areas composed of cortical type epithelium. Hence a medullary microenvironment with epidermoid cells corresponding to Hassall's corpuscules seems to be necessary for specific intrathymic B-cell homing.

胸腺上皮肿瘤中的b细胞。上皮内和上皮外b细胞区室的免疫组织化学分析。
采用免疫组化方法对26例胸腺瘤和胸腺癌进行了肿瘤内b细胞的存在和分布研究。双染色实验显示胸腺上皮肿瘤中存在两种不同的b细胞群。一个位于血管周围间隙(PVS)内,由基底膜与肿瘤上皮隔开。在所有肿瘤中,PVS均含有外周b细胞免疫表型的淋巴细胞。大量具有生发中心形成的b细胞几乎只在重症肌无力(MG)相关肿瘤中发现,主要在皮质胸腺瘤和高分化胸腺癌中发现。第二群b细胞位于肿瘤上皮网中,主要位于以表皮样细胞或Hassall小体为特征的类器官髓质分化区。该群体通常由具有树突状特征的大型CD23+细胞组成,类似于正常人胸腺的特殊类型的髓内b细胞。相比之下,b细胞在表现梭形细胞髓样分化的混合性胸腺瘤中不常见,在由皮质型上皮组成的肿瘤区几乎完全不存在。因此,具有表皮样细胞的髓质微环境似乎是胸腺内特异性b细胞归巢所必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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