Structure and antigenic properties of Citrobacter freundii lipopolysaccharides.

The Journal of applied bacteriology Pub Date : 1993-05-01
H Chart, G A Willshaw, T Cheasty, B Rowe
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Abstract

Citrobacter freundii strain E69366 was detected in a colony immunoassay with a rabbit antiserum prepared to a strain of Escherichia coli belonging to serogroup O157. Lipopolysaccharide (LPS) was shown to contain the epitope(s) involved in antibody binding. LPS prepared from strain E69366, with hot phenol contained core-LPS that migrated to the water-phase and long-chain LPS that separated into the phenol-phase. A total of 36 strains of Cit. freundii were analysed for their LPS profiles by SDS-PAGE. Sixteen could be allocated into five groups (A(3), B(7), C(2), D(2) and E(2)) based on similarities in LPS profile. The remaining strains either expressed unique SDS-PAGE profiles (18) or did not express long-chain LPS (2). All strains with a profile 'A' reacted with the antiserum prepared to E. coli O157, whereas those with other profiles did not. The two strains of Cit. freundii expressing LPS profiles designated 'E' reacted with an antiserum prepared in rabbits to E. coli O45.

弗氏柠檬酸杆菌脂多糖的结构与抗原性。
对大肠杆菌O157血清群制备的兔抗血清进行菌落免疫分析,检测到弗氏柠檬酸杆菌E69366。脂多糖(LPS)被证明含有参与抗体结合的表位。从菌株E69366制备LPS,其中热酚含核心LPS迁移到水相,长链LPS分离到酚相。采用SDS-PAGE分析了36株弗氏线虫的LPS谱。根据LPS谱相似性,将16只小鼠分为A(3)、B(7)、C(2)、D(2)、E(2) 5组。其余菌株要么表达独特的SDS-PAGE谱(18),要么不表达长链LPS(2)。所有谱为“a”的菌株都能与大肠杆菌O157制备的抗血清反应,而其他谱为“a”的菌株则不能。这两株表达LPS标记为“E”的citt . freundii菌株与兔制备的抗血清对大肠杆菌O45发生反应。
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