Aneuploidy in human lymphocytes: an extensive study of eight individuals of various ages

Mutation Research/DNAging Pub Date : 1993-03-01 DOI:10.1016/0921-8734(93)90003-L

Florence Richard , Alain Aurias , Jérôme Couturier , Anne-Marie Dutrillaux , Anne Flüry-Hérard , Michèle Gerbault-Seureau , Françoise Hoffschir , Eugénie Lamoliatte , Danielle Lefrançois , Martine Lombard , Martine Muleris , Marguerite Prieur , Michèle Ricoul , Laure Sabatier , Evani Viegas-Péquignot , Vitaly Volobouev , Bernard Dutrillaux

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引用次数: 60

Abstract

Data on aneuploidy from a prospective study on a large number of lymphocyte metaphases (over 1000 in 72-h and 100 in 48-h cultures) per individual from eight healthy donors of various ages are reported. Chromosome losses were dependent on culture time, being significantly more frequent in 72-h than in 48-h cultures. All donors exhibited various degrees of aneuploidy which increased with age in women. This increase resulted essentially from X chromosome losses, as previously reported. Although the rate of aneuploidy limited to autosomes was similar in newborns and in adults, the distributions of the missing autosomes were different. In the two newborns studied, autosome aneuploidy was random. In the adults, a significant inverse correlation with autosome lenghts was observed. The inverse correlation between chromosome lenghts and losses may be explained by selective pressure against monosomic cells in the adults.

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人类淋巴细胞的非整倍体:对8个不同年龄个体的广泛研究

本文报道了来自8个不同年龄的健康供体的大量淋巴细胞中期(72小时培养超过1000个，48小时培养100个)的前瞻性研究的非整倍性数据。染色体丢失与培养时间有关，72h的染色体丢失明显高于48h。所有供体均表现出不同程度的非整倍体，随年龄增长而增加。如前所述，这种增加主要是由于X染色体的丢失。虽然新生儿和成人的常染色体非整倍体率相似，但缺失常染色体的分布却不同。在研究的两个新生儿中，常染色体非整倍体是随机的。在成人中，观察到与常染色体长度显著负相关。染色体长度与染色体损失之间的负相关关系可能是由于成人对单体细胞的选择压力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mutation Research/DNAging

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