Aspergillus nidulans Mutants Affected in Acetate Metabolism Isolated as Lipid Nonutilizers

Laura Kawasaki, Amelia Farrés, Jesús Aguirre
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引用次数: 17

Abstract

Kawasaki, L., Farrés, A., and Aguirre, J. 1995. Aspergillus nidulans mutants affected in acetate metabolism isolated as lipid nonutilizers. Experimental Mycology 19, 81-85. Interest in extracellular fungal lipases has increased mainly because of their industrial applications. However, no studies have been done on a genetically well characterized filamentous fungus like Aspergillus nidulans. Here we show that A. nidulans produces an extracellular lipase when grown in solid or liquid cultures containing lipids as carbon source. This lipase is glucose-repressed in a creA-independent fashion. Seven mutants isolated by their inability to utilize lipids as sole carbon source were also unable to utilize acetate as sole carbon source. Representative mutants from each of three complementation groups were tested for allelism with strains carrying well known mutations affecting acetate metabolism. They were found to contain acuD (Isocitrate lyase), acuF (PEP carboxykinase), and acuE (Malate synthase) alleles. Screening of lipid nonutilizing mutants for growth in acetate provides a method for the isolation of both lipase minus and new acetate metabolism mutants.

非脂质利用菌对醋酸代谢的影响
Kawasaki, L., farrsamas, A.和Aguirre, J. 1995。中性曲霉突变体对脂质代谢的影响。实验真菌学,19,81-85。对细胞外真菌脂肪酶的兴趣增加主要是因为它们的工业应用。然而,目前还没有对一种具有良好遗传特征的丝状真菌进行研究,比如细粒曲霉。在这里,我们表明,当在含有脂质作为碳源的固体或液体培养物中生长时,芽孢杆菌产生细胞外脂肪酶。这种脂肪酶以一种不依赖于氨基酸的方式抑制葡萄糖。7个不能利用脂质作为唯一碳源的突变体也不能利用醋酸作为唯一碳源。来自三个互补组的代表性突变体与携带影响乙酸代谢的已知突变的菌株进行等位基因检测。发现它们含有acuD(异柠檬酸裂解酶),acuF (PEP羧激酶)和acuE(苹果酸合成酶)等位基因。筛选脂质不利用突变体在乙酸中生长,为分离脂肪酶缺失突变体和新的醋酸代谢突变体提供了一种方法。
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