S-acetyl- and S-phenylacetyl-glutathione as glutathione precursors in rat plasma and tissue preparations.

Enzyme & protein Pub Date : 1994-01-01 DOI:10.1159/000474975
L Galzigna, V Rizzoli, P Schiappelli, C Moretto, A Bernareggi
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引用次数: 7

Abstract

S-acetyl- and S-phenylacetyl-glutathione derivatives were synthesized by using a new procedure. The derivatives were incubated with rat plasma and red blood cells, and also with cytosol from rat liver, kidney and heart, or tissue slices from rat heart, kidney and liver. A limited hydrolysis of the compounds occurs in plasma, whereas hydrolysis occurs to a larger extent in tissue cytosols. Both purified and crude gamma-glutamyl-transpeptidase from different sources recognized the S-acetyl- and S-phenylacetyl derivatives as substrates. Intracellular glutathione increases after incubating the derivatives with red blood cells. A potential role of S-acetyl- and S-phenylacetyl-glutathione in replenishing cells with exogenous glutathione is envisaged.

大鼠血浆和组织制剂中s -乙酰和s -苯基乙酰谷胱甘肽作为谷胱甘肽前体。
采用新工艺合成了s -乙酰谷胱甘肽和s -苯基乙酰谷胱甘肽衍生物。衍生物与大鼠血浆、红细胞、大鼠肝、肾、心细胞质或大鼠心、肾、肝组织切片孵育。化合物的有限水解发生在血浆中,而水解在组织细胞质中发生更大程度。来自不同来源的纯化和粗γ -谷氨酰转肽酶都能识别s -乙酰基和s -苯乙酰基衍生物作为底物。与红细胞孵育后细胞内谷胱甘肽增加。设想了s -乙酰和s -苯基乙酰谷胱甘肽在补充外源性谷胱甘肽细胞中的潜在作用。
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