Baseline and mitomycin C(MMC)-induced sister-chromatid exchanges in XX and XY cells of Callithrix jacchus chimeric twins

Mutation Research Letters Pub Date : 1995-08-01 DOI:10.1016/0165-7992(95)00035-6

M.P. Bigatti , L. Lambert , S. Crovella , G. Ardito

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引用次数: 1

Abstract

Lymphocytes from chimetric individuals of the species Callithrix jacchus (Primates) were examined to evaluate differences in the frequency of sister chromatid exchanges (SCE) between XX and XY cells. The aim was to discover whether SCE differ according to genetic sex and whether XX and XY cells show a different sensitivity to SCE inducing agents. This experimental model has enabled us to eliminate the possible differences caused by environmental factors. The results obtained do not reveal significant differences between male and female cells, in either the baseline SCE frequency or that induced by mitomycin C at concentrations of 0.01 and 0.03 μg/ml. No signficant differences were observed in the distribution of high SCE frequency cells (HFC), even if it is possible to observe a higher level of exchanges in XX cells in each trial. With regard to the phenotypic sex, there appears to be a trend towards slightly higher SCE rates in females, even if results are not statistically significant.

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基线和丝裂霉素C(MMC)诱导的紫丝茅嵌合双胞胎XX和XY细胞的姐妹染色单体交换

本文研究了灵长类Callithrix jacchus (Callithrix jacchus，灵长类动物)XX和XY细胞姊妹染色单体交换(SCE)频率的差异。目的是发现SCE是否因遗传性别而不同，以及XX和XY细胞对SCE诱导剂的敏感性是否不同。这个实验模型使我们能够消除环境因素可能造成的差异。结果显示，无论是基线SCE频率还是丝裂霉素C在0.01和0.03 μg/ml浓度下诱导的SCE频率，男性和女性细胞之间都没有显著差异。高SCE频率细胞(HFC)的分布没有观察到显著差异，即使在每次试验中有可能观察到XX细胞的更高水平的交换。就表现型性别而言，女性的SCE发生率似乎有略高的趋势，即使结果没有统计学意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Mutation Research Letters

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