Analysis of mouse hemoglobin alpha-chain locus mutation induced by N-ethyl-N-nitrosourea.

Idengaku zasshi Pub Date : 1995-08-01 DOI:10.1266/jjg.70.497
T Murota
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引用次数: 2

Abstract

In a mouse specific-locus test in stem-cell spermatogonia of mice after treatment with N-ethyl-N-nitrosourea (ENU), a mutation at the hemoglobin alpha-chain locus was obtained. In an original mutant, the mutation was detected as a change in band density using cellulose acetate electrophoresis, e.g. the slow moving band was much higher than that of normal F1 mice. Yet, migration of the mouse bands was similar. In isoelectric focusing, the migration of a band toward cathode was observed, indicating that the variant hemoglobin is caused by mutation at an alpha-globin chain. This mutation was found to be heritable and viable in homozygous condition from a genetic test on the mutant, suggesting that this mutation would be due to a base-pair change. According to the usual nomenclature of mutation at the hemoglobin locus, the allele symbol of this mutation was tentatively designated as Hbacc3.

n -乙基-n -亚硝基脲致小鼠血红蛋白α链位点突变分析。
用n -乙基-n -亚硝基脲(ENU)对小鼠干细胞精原细胞进行小鼠特异性位点检测,发现血红蛋白α链位点发生突变。在原始突变体中,通过醋酸纤维素电泳检测到突变条带密度的变化,例如慢移动条带比正常F1小鼠高得多。然而,小鼠带的迁移是相似的。在等电聚焦中,观察到一个带向阴极的迁移,表明变异血红蛋白是由α -珠蛋白链突变引起的。通过对突变体的基因测试,发现该突变具有遗传性,并且在纯合子条件下存活,这表明该突变可能是由于碱基对的改变。根据血红蛋白位点突变的常用命名法,该突变的等位基因符号暂定为Hbacc3。
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