Immunoreactive atrial and brain natriuretic peptides are co-localized in Purkinje fibres but not in the innervation of the bovine heart conduction system.

The Histochemical Journal Pub Date : 1995-03-01
M Hansson, S Forsgren
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Abstract

Recently, we observed that atrial natriuretic peptide (ANP) immunoreactivity was present in Purkinje fibres and nerve fibre varicosities in the conduction system of the bovine heart. In order to elucidate further the morphological correlation between natriuretic peptides and the conduction system, the distribution of brain natriuretic peptide (BNP) was examined. The different parts of the conduction system in the bovine heart were dissected out and processed for immunohistochemistry with antisera against BNP and ANP. BNP immunoreactivity was frequently observed in Purkinje fibres of the atrioventricular bundle, whereas only a few Purkinje fibres in the ventricular part of the conduction system showed immunoreaction. BNP immunoreactivity was detected in regions of the Purkinje fibres which also showed ANP immunoreactivity. BNP immunoreactivity was not observed in nerve fibre varicosities. Methodologically, a larger number of small BNP immunofluorescent granular structures was observed by using an elution-restaining technique instead of conventional immunohistochemistry. The present study shows that BNP and ANP immunoreactivities frequently occur in the atrioventricular bundle and that they are co-localized in Purkinje fibres, but not in nerve fibre varicosities, in the conduction system. As previously has been proposed for ANP, the present observations suggest that also BNP may act in an autocrine and/or paracrine way in the conduction cells.

免疫反应性心房和脑利钠肽在浦肯野纤维中共定位,但不在牛心脏传导系统的神经支配中。
最近,我们观察到心房利钠肽(ANP)免疫反应性存在于牛心脏传导系统的浦肯野纤维和神经纤维静脉曲张。为了进一步阐明钠肽与传导系统的形态学关系,对脑钠肽(BNP)的分布进行了检测。解剖牛心脏传导系统的不同部位,用抗BNP和ANP血清进行免疫组化处理。在房室束浦肯野纤维中经常观察到BNP免疫反应,而在传导系统的心室部分只有少数浦肯野纤维表现出免疫反应。在浦肯野纤维区域检测到BNP免疫反应性,同时也显示ANP免疫反应性。神经纤维静脉曲张未观察到BNP免疫反应性。在方法上,通过使用洗脱-保留技术而不是传统的免疫组织化学,观察到大量的小BNP免疫荧光颗粒结构。目前的研究表明,BNP和ANP免疫反应经常发生在房室束,它们在传导系统的浦肯野纤维中共定位,但在神经纤维曲张中不存在。正如先前提出的ANP一样,目前的观察表明,BNP也可能以自分泌和/或旁分泌的方式在传导细胞中起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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