Increased cytokine levels and abnormal response of myeloid progenitor cells to granulocyte colony-stimulating factor in a case of severe congenital neutropenia. In vitro effects of stem cell factor.

T Shitara, H Ijima, S Yugami, M Sotomatu, T Kuroume
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Abstract

Purpose: The cytokine levels and the in vitro granulopoiesis were studied to evaluate the mechanism of impaired granulopoiesis in severe congenital neutropenia (SCN).

Patient and methods: The patient was a 5-year-old boy with SCN. We assayed the colony-stimulating activity (CSA) produced by peripheral blood (PB) cells from the patient. The plasma levels of cytokines were measured using enzyme immunoassay. These included granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 1 alpha (IL-1 alpha), IL-1 beta, IL-2, IL-3, IL-4, IL-6, and tumor necrosis factor-alpha. The effects of IL-3 and stem cell factor (SCF) on the proliferation of granulocyte-macrophage colony-forming cells (GM-CFCs) were studied.

Results: CSA produced by PB cells from the patient was almost the same as in the healthy control. The level of endogenous G-CSF was elevated to 334 pg/ml, and GM-CSF, IL-2, IL-3, and IL-6 were slightly elevated. The numbers of GM-CFCs were markedly depressed in the presence of G-CSF alone and showed no increment on additional stimulation by IL-3. SCF in combination with G-CSF significantly augmented the proliferation of GM-CFCs.

Conclusions: These findings suggest that some cytokines including G-CSF may be elevated in SCN patients and that CSF may play an important role in the pathogenesis of SCN.

严重先天性中性粒细胞减少症1例中细胞因子水平升高及髓系祖细胞对粒细胞集落刺激因子的异常反应。干细胞因子在体外的作用。
目的:研究重度先天性中性粒细胞减少症(SCN)患者细胞因子水平和体外粒细胞生成的变化,探讨其粒细胞生成受损的机制。患者和方法:患者为5岁男童,伴有SCN。我们检测了患者外周血细胞产生的集落刺激活性(CSA)。采用酶免疫分析法测定血浆细胞因子水平。包括粒细胞集落刺激因子(G-CSF)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素1 α (IL-1 α)、IL-1 β、IL-2、IL-3、IL-4、IL-6和肿瘤坏死因子α。研究了IL-3和干细胞因子(SCF)对粒细胞-巨噬细胞集落形成细胞(GM-CFCs)增殖的影响。结果:患者PB细胞产生的CSA与健康对照组基本相同。内源性G-CSF水平升高至334 pg/ml, GM-CSF、IL-2、IL-3、IL-6轻度升高。G-CSF单独存在时,GM-CFCs的数量明显下降,IL-3的额外刺激没有增加。SCF与G-CSF联合可显著增强gm - cfc的增殖。结论:这些发现提示SCN患者中包括G-CSF在内的一些细胞因子可能升高,CSF可能在SCN的发病机制中起重要作用。
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