Utility of Argyrophilic nucleolar organizer regions (AgNOR) in differentiating malignant from benign of colorectal tumors.

L T Chen, M S Huang, Y L Wang, C S Liu, C M Jan
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Abstract

Background: Argyrophilic nucleolar organizer regions (AgNOR) can reflect the activity of cellular proliferation. The purpose of this study is to evaluate the potential value of AgNOR in differentiating benign from malignant colon epithelial neoplasms, and to determine the correlation between the nucleus AgNOR and the grade of colonic adenocarcinomas.

Methods: In this study, AgNOR technique was applied to 61 paraffin embedded sections of colorectal tissue including normal mucosa (n = 10), adenomatous polyp (n = 16), and adenocarcinoma (n = 35).

Results: The mean +/- standard error (SE) numbers of AgNOR dots per nucleus of normal mucosa, adenomatous polyp, and adenocarcinoma were 2.17 +/- 0.07 (n = 10) 3.89 +/- 0.10 (n = 16) and 5.52 +/- 0.10 (n = 35), respectively (p < 0.00001). In addition, the mean numbers of AgNOR dots per nucleus of well differentiated (WD) adenocarcinoma (n = 14), moderately differentiated (MD) adenocarcinoma (n = 11) and poorly differentiated (PD) adenocarcinoma (n = 10) were 5.20 +/- 0.12, 5.81 +/- 0.20, 5.67 +/- 0.15, respectively. MD and PD tumor had significantly higher AgNOR count than that of WD tumor (p < 0.05). However, there was no significant difference between MD and PD colorectal adenocarcinoma.

Conclusion: AgNOR method is a simple, rapid method in diagnosis of colorectal tumors, and it provides a useful adjunct to histopathology in the diagnosis of colorectal tumors.

嗜银核仁组织区(AgNOR)在鉴别结直肠肿瘤良恶性中的应用。
背景:嗜银核仁组织区(AgNOR)可以反映细胞的增殖活性。本研究的目的是评估AgNOR在鉴别结肠上皮良恶性肿瘤中的潜在价值,并确定AgNOR核与结肠腺癌分级的相关性。方法:本研究采用AgNOR技术对61例结直肠组织石蜡包埋切片,包括正常粘膜(n = 10)、腺瘤性息肉(n = 16)和腺癌(n = 35)。结果:正常粘膜、腺瘤性息肉和腺癌每核AgNOR点的平均+/-标准误差(SE)分别为2.17 +/- 0.07 (n = 10)、3.89 +/- 0.10 (n = 16)和5.52 +/- 0.10 (n = 35),差异有统计学意义(p < 0.00001)。此外,高分化(WD)腺癌(n = 14)、中分化(MD)腺癌(n = 11)和低分化(PD)腺癌(n = 10)每核平均AgNOR点数分别为5.20 +/- 0.12、5.81 +/- 0.20、5.67 +/- 0.15。MD和PD肿瘤的AgNOR计数明显高于WD肿瘤(p < 0.05)。然而,MD和PD之间的结直肠腺癌无显著差异。结论:AgNOR法是一种简便、快速的结直肠肿瘤诊断方法,为结直肠肿瘤的组织病理学诊断提供了有益的辅助手段。
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