A molecular study of ocular herpes simplex infection in Kaohsiung, Taiwan. Part I: Cleavage pattern analysis.

H L Wang, M M Sheu, W L Huang, C W Chen, L C Chou, K H Lin
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Abstract

In order to investigate the relationship between genotypes of herpes simplex virus (HSV) and its clinical pictures in herpes simplex virus keratoconjunctivitis (HSK), fifty nine strains of HSV isolates, collected in the Kaohsiung Medical College Hospital during January 1981 to July 1991, and F strain (ATCC VR-73), a standard HSV-1, were studied. The viruses were isolated from HSK patients. All 59 strains were identified as HSV-1 using immunofluorescence monoclonal antibody method. The HSV DNAs were extracted and digested by restriction enzymes, BamH I, Kpn I and Sal I, followed by electrophoresis, and photographing. Six kinds of DNA cleavage patterns were found in Taiwan. The majority were E subtype (56.4%) then A subtype (27.3%), D subtype (7.3%), F subtype (5.4%), G subtype (1.8%) and H subtype (1.8%). Subtypes B and C were not found in this study. The cleavage patterns of two recurrent cases belonged to E and A subtypes. The identical cleavage patterns of two bilateral infections all belonged to the E subtype.

台湾高雄地区眼部单纯疱疹感染的分子研究。第一部分:解理模式分析。
为探讨单纯疱疹病毒(HSV)基因型与其临床表现的关系,本研究于1981年1月至1991年7月在高雄医学院附属医院采集59株单纯疱疹病毒(HSV)分离株,并以标准的1型单纯疱疹病毒F株(ATCC vr73)为研究对象。这些病毒是从HSK患者中分离出来的。采用免疫荧光单克隆抗体法鉴定59株均为HSV-1型。提取HSV dna,用限制性内切酶、BamH I、Kpn I和Sal I酶切,电泳、拍照。在台湾发现了6种不同的DNA切割模式。以E亚型(56.4%)、A亚型(27.3%)、D亚型(7.3%)、F亚型(5.4%)、G亚型(1.8%)和H亚型(1.8%)居多。本研究未发现B和C亚型。2例复发病例的卵裂模式分别为E和A亚型。两例双侧感染的切割模式相同,均属于E亚型。
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