Perfusion-fixation of the heart and its conduction system for ultrastructural studies.

European journal of cardiology Pub Date : 1980-02-01
M K Thapar, W W Johnson, S K Sanyal
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引用次数: 0

Abstract

Nine hearts from children who died from noncardiac causes were perfused within 3 h of death with 2.5% glutaraldehyde, 0.1 M sodium cacodylate, pH 7.2, at 4 degrees C. Each heart was perfused continuously for 4 h using a perfusion pressure of 110 mm Hg. The ultrastructural characteristics of the perfused atrial and ventricular myocardium and conduction system, including sinoatrial, atrioventricular node and Purkinje fibers, were studied. These observations were compared with standard published observations on similar tissues. The results demonstrate that the present method yields comparable results and hence provides a simple, yet effective, means for studying ultrastructural aspects of the heart and its conduction system in children. In addition, this method permits satisfactory gross morphologic examination of the organ.

心脏灌注固定及其传导系统的超微结构研究。
对9例非心源性死亡患儿在死亡后3 h内灌注2.5%戊二醛、0.1 M草酸钠、pH 7.2、4℃,在110 mm Hg灌注压力下连续灌注4 h,观察灌注后心房和心室心肌及传导系统(包括窦房、房室结和浦肯野纤维)的超微结构特征。将这些观察结果与发表在类似组织上的标准观察结果进行比较。结果表明,目前的方法产生了可比的结果,因此提供了一种简单而有效的方法来研究儿童心脏及其传导系统的超微结构方面。此外,这种方法可以对器官进行令人满意的大体形态学检查。
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