Determination of brain regional normetanephrine levels by liquid chromatography with electrochemical detection (LC-EC)

Progress in neuro-psychopharmacology Pub Date : 1981-01-01 DOI:10.1016/0364-7722(81)90046-1

Andrew S. Chiu , Damodar D. Godse, Jerry J. Warsh

引用次数: 4

Abstract

1.
1. A liquid chromatography-electrochemical detection (LC-EC) assay for brain normetanephrine (NMN) has been developed which also allows concurrent determination of brain norepinephrine (NE), dopamine (DA) and serotonin (5-HT) concentrations.
2.
2. Tissue samples were prepurified by cation exchange chromatography and the amines then separated by reversed-phase liquid chromatography on an octylsilane stationary phase.
3.
3. This method permits measurement of at least 200 pg of the above amines in brain.
4.
4. The concentrations of NMN in rat and mouse brain regions were found to be in the range of 0.115–0.435 ng/mg protein.

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液相色谱-电化学检测(LC-EC)测定脑区去甲肾上腺素水平

1.1. 建立了脑去甲肾上腺素(NMN)的液相色谱-电化学检测(LC-EC)方法，该方法还可以同时测定脑去甲肾上腺素(NE)、多巴胺(DA)和血清素(5-HT)的浓度。用阳离子交换色谱法对组织样品进行预纯化，然后在辛烷基硅烷固定相上用反相液相色谱法分离胺。这种方法允许在大脑中测量至少200pg以上胺。大鼠和小鼠脑区NMN浓度在0.115 ~ 0.435 ng/mg蛋白之间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Progress in neuro-psychopharmacology

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