Purification and properties of aldose 1-epimerase from aspergillus niger

Abstract

Aspergillus niger ATCC 6274 was selected as an aldose 1-epimerase (EC 5.1.3.3) producer from 45 stock cultures of A. niger. The aldose 1-epimerase was purified 115-fold to apparent homogeneity from cell extracts with a yield of 2.6%. The molecular weight was calculated to be 260 000 and that of the subunit to be 130 000. The enzyme preparation was active at pH 5–7. The K_m value was 50 mM and the V value was 1200 units/mg toward α-d-glucose. This enzyme catalyzed mutarotation of the following substrates; α-d-glucose, β-d-fructose, β-l-arabinose and β-d-galactose. The time required for glucose determination with a glucose oxidase reagent was significantly shortened by the addition of aldose 1-epimerase.