Interaction of bovine serum albumin with PGBx (polymeric 15-keto-prostaglandin B1).

Abstract

Bovine serum albumin (BSA) interacts with PGBx, a polymeric derivative of 15-keto-prostaglandin B1, to form a complex that does not exhibit the fluorescence of free BSA. The complex is soluble at pH 5.2 in contrast to free PGBx, which is insoluble. Molar ratio of the BSA-PGBx complex is 1:18. This complexing appears to suppress the ability shown by non-complexed PGBx to reactivate phosphorylation in degraded isolated rat liver mitochondria.