Lectin-induced suppression of antibody-dependent cellular cytotoxicity (ADCC) of human peripheral blood mononuclear cells.

P Gergely, I Láng, L Kalmár, R González-Cabello
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Abstract

Incubation of human peripheral blood mononuclear cells in the presence of 25 mg/l concanavalin A (Con A) or 2 mg/l phytohaemagglutinin (PHA) suppressed their ADCC activity. Thirty-minute incubation with the mitogens resulted in a significant decrease in ADCC activity. The effect was more striking with longer (24 and 48 h) incubation. The suppressive effect of PHA was abolished after 6 days incubation, while no such phenomenon was observed with Con A. Macrophages participating in the ADCC reaction were not influenced by the lectin treatment, though their removal increased the suppressive effect. The lectin-induced suppression of ADCC activity did not correlate with the suppression of Con A-induced blastogenesis. The suppressive effect of lectins on ADCC is not mediated through suppressor cells, but rather represents a direct action of ligands on the (killer) lymphocyte membranes, resulting probably in an altered metabolism, or inhibition of membrane mobility or lymphocyte locomotion.

凝集素诱导的人外周血单个核细胞抗体依赖性细胞毒性(ADCC)抑制。
人外周血单个核细胞在25 mg/l豆蛋白A (Con A)或2 mg/l植物血凝素(PHA)存在下的孵育抑制了它们的ADCC活性。与有丝分裂原孵育30分钟,ADCC活性显著降低。孵育时间越长(24和48小时),效果越显著。PHA的抑制作用在孵育6天后消失,而Con a则没有这种现象。参与ADCC反应的巨噬细胞不受凝集素处理的影响,但它们的去除增加了抑制作用。凝集素诱导的ADCC活性抑制与Con a诱导的胚发生抑制不相关。凝集素对ADCC的抑制作用不是通过抑制细胞介导的,而是代表了配体对(杀伤)淋巴细胞膜的直接作用,可能导致代谢改变,或膜运动或淋巴细胞运动的抑制。
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