Potential of direct immersion solid-phase microextraction to characterize dissolved volatile organic compounds released by submerged decaying rat cadavers
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引用次数: 2
Abstract
The decomposition process involves the degradation of carbohydrates, nucleic acids, proteins and fats, and leads to the release of volatile organic compounds (VOCs) among many other decomposition by-products. Despite the extensive literature on the VOCs emitted in the air from vertebrate corpses, there is a lack of research dedicated to aquatic decomposition. In this study, we aimed to evaluate the potential of direct immersion solid-phase microextraction gas chromatography coupled with mass spectrometry (DI-SPME/GC/MS) to characterize dissolved cadaveric VOCs. Dimethyl disulphide and indole -two compounds commonly released during decomposition- were selected to evaluate and set the optimal methodological parameters, which were found to be 10 min of collection performed under 27.5 °C and a stirring rate of 250 rpm. Using responsive surface methodology, the obtained curves highlighted the appropriate conditions for the dissolved cadaveric volatilome analysis. The method allows to trap 17 dissolved cadaveric VOCs, including commonly encountered compounds such as dimethyl disulfide, 9-hexanoic acid, dimethyl trisulfide and indole. DI-SPME/GC/MS has therefore potential for the identification of dissolved cadaveric VOCs, pending further tests are performed to optimize the method and make it capable of detecting all dissolved VOCs, through all stages of decomposition.
期刊介绍：
Forensic Chemistry publishes high quality manuscripts focusing on the theory, research and application of any chemical science to forensic analysis. The scope of the journal includes fundamental advancements that result in a better understanding of the evidentiary significance derived from the physical and chemical analysis of materials. The scope of Forensic Chemistry will also include the application and or development of any molecular and atomic spectrochemical technique, electrochemical techniques, sensors, surface characterization techniques, mass spectrometry, nuclear magnetic resonance, chemometrics and statistics, and separation sciences (e.g. chromatography) that provide insight into the forensic analysis of materials. Evidential topics of interest to the journal include, but are not limited to, fingerprint analysis, drug analysis, ignitable liquid residue analysis, explosives detection and analysis, the characterization and comparison of trace evidence (glass, fibers, paints and polymers, tapes, soils and other materials), ink and paper analysis, gunshot residue analysis, synthetic pathways for drugs, toxicology and the analysis and chemistry associated with the components of fingermarks. The journal is particularly interested in receiving manuscripts that report advances in the forensic interpretation of chemical evidence. Technology Readiness Level: When submitting an article to Forensic Chemistry, all authors will be asked to self-assign a Technology Readiness Level (TRL) to their article. The purpose of the TRL system is to help readers understand the level of maturity of an idea or method, to help track the evolution of readiness of a given technique or method, and to help filter published articles by the expected ease of implementation in an operation setting within a crime lab.
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