{"title":"[Daily rhythms of melatonin in pineal, serum and urine of rats (author's transl)].","authors":"R Alonso, Y Ozaki, R J Wurtman","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The levels of melatonin in urine, serum, and pineals were determined in rats during the light/dark cycle. Previous to the radioimmunoassay, melatonin was isolated using a new ascending chromatographic method with programmed multiple development. The daily excretion of melatonin and its serum levels showed a characteristic rhythm, night samples containing several times more melatonin than those of the light period. The pineal content of melatonin of animals killed at different intervals also showed a similar day/night rhythm. The chromatographic method used gave a perfect separation of melatonin from other materials which could interfere in the assay. The mobility of material detected by RIA was identical to that of authentic melatonin chromatographed concurrently.</p>","PeriodicalId":76418,"journal":{"name":"Reproduccion","volume":"4 3","pages":"225-33"},"PeriodicalIF":0.0000,"publicationDate":"1980-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproduccion","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The levels of melatonin in urine, serum, and pineals were determined in rats during the light/dark cycle. Previous to the radioimmunoassay, melatonin was isolated using a new ascending chromatographic method with programmed multiple development. The daily excretion of melatonin and its serum levels showed a characteristic rhythm, night samples containing several times more melatonin than those of the light period. The pineal content of melatonin of animals killed at different intervals also showed a similar day/night rhythm. The chromatographic method used gave a perfect separation of melatonin from other materials which could interfere in the assay. The mobility of material detected by RIA was identical to that of authentic melatonin chromatographed concurrently.
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