Uroporphyrinogen-I-synthetase activity in red blood cells of lead-exposed workers.

A El-Waseef
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Abstract

Lead-exposed (n = 26) and control (n = 12) subjects were investigated for their blood lead concentration erythrocyte 5-amino-laevulinic acid dehydratase (5-ALAD) and erythrocyte uroporphyrinogen-I-synthetase (URO-I-S) activity; 5-amino-laevulinic acid (5-ALA) and porphobilinogen (PBG) were used as substrates in the synthetase assay. In the lead workers erythrocyte 5-ALA dehydratase was grossly inhibited but with PBG as substrate the synthetase activity was not significantly different from the control group. With 5-ALA as substrate the synthetase assay showed marked inhibition. Addition of zinc (0.1 mmol/l) and dithiotheritol (0.5 mmol/l) brought the activities of both the dehydratase and synthetase (using 5-ALA as substrate) back into the ranges seen in the control group. With porphobilinogen as substrate higher concentrations of zinc caused inhibition of the synthetase, whilst reduction of added zinc to 0.01 mmol/l resulted in stimulation of the synthetase. A good correlation (r = 0.87) was obtained in synthetase assay when PBG and 5-aminolaevulinate (with added zinc and dithiothreitol) were used as substrates. With these additions 5-ALA may be used as a substrate in the URO-I-S assay in the investigation of latent cases of acute intermittent porphyria.

铅暴露工人红细胞中尿卟啉原- i合成酶活性。
研究了铅暴露组(26例)和对照组(12例)血铅浓度、红细胞5-氨基乙酰丙酸脱水酶(5-ALAD)和红细胞尿卟啉原- 1合成酶(urop - 1 -s)活性;合成酶实验以5-氨基乙酰丙酸(5-ALA)和卟啉胆色素原(PBG)为底物。铅工人红细胞5-ALA脱水酶被严重抑制,但以PBG为底物的合成酶活性与对照组无显著差异。以5-ALA为底物的合成酶实验显示明显的抑制作用。添加锌(0.1 mmol/l)和二硫醚醇(0.5 mmol/l)使脱水酶和合成酶(以5-ALA为底物)的活性恢复到对照组的范围。以卟胆色素原为底物,较高浓度的锌对合成酶有抑制作用,而将锌添加量降至0.01 mmol/l则对合成酶有刺激作用。以PBG和5-氨基乙酰戊酸盐(添加锌和二硫苏糖醇)为底物,在合成酶实验中获得了良好的相关性(r = 0.87)。有了这些添加物,5-ALA可以作为底物用于急性间歇性卟啉症潜伏病例调查中的eu - i - s测定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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