[3H-Methyl]-methionine as possible methyl donor for formation of 5'-terminus of in vitro synthesized mRNA of cytoplasmic polyhedrosis virus of silkworm Bombyx mori.

Scientia Sinica Pub Date : 1981-12-01
A Z Wu, R M Dai, X R Shen, Y K Sun
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Abstract

The mRNA of the cytoplasmic polyhedrosis virus of silkworm Bombyx mori could be synthesized in vitro through the action of the virion associated RNA-polymerase in the presence of [3H-methyl]-methionine instead of S-adenosyl-L-methionine. The 3H-mRNA is isolated from the reaction mixture through a column of DEAE-Sephadex A-25 and digested with nuclease p1 and snake venom phosphodiesterase. The results of paper electrophoresis show that the methyl group of methionine is incorporated into the 5'-terminus of CPV mRNA. Even in the presence of S-adenosyl-L-methionine the [3H-methyl]-methionine could still function as a methyl donor for the formation of the 5'-caps of the mRNA of CPV.

[3h -甲基]-蛋氨酸作为家蚕细胞质多角体病毒体外合成mRNA 5′端形成的可能甲基供体
家蚕胞质多角体病毒在[3h -甲基]-蛋氨酸的存在下,通过病毒粒子相关rna -聚合酶的作用,可以在体外合成[3h -甲基]-蛋氨酸而不是s -腺苷- l-蛋氨酸。通过DEAE-Sephadex a -25柱从反应混合物中分离3H-mRNA,用核酸酶p1和蛇毒磷酸二酯酶消化。纸电泳结果显示,蛋氨酸的甲基被纳入CPV mRNA的5'端。即使在s -腺苷- l-蛋氨酸存在的情况下,[3h -甲基]-蛋氨酸仍然可以作为甲基供体形成CPV mRNA的5'-cap。
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