Primary structure of an acidic ribosomal protein YPA1 from Saccharomyces cerevisiae

Takuzi Itoh
{"title":"Primary structure of an acidic ribosomal protein YPA1 from Saccharomyces cerevisiae","authors":"Takuzi Itoh","doi":"10.1016/0005-2795(81)90088-X","DOIUrl":null,"url":null,"abstract":"<div><p>The complete primary structure of an acidic ribosomal protein YPA1 from <em>Saccharomyces cerevisiae</em> has been determined. YPA1 is composed of 110 amino acid residues and has the composition: Asp<sub>7</sub>, Asn<sub>2</sub>, Thr<sub>2</sub>, Ser<sub>9</sub>, Glu<sub>15</sub>, Gln<sub>2</sub>, Pro<sub>3</sub>, Gly<sub>15</sub>, Ala<sub>21</sub>, Val<sub>6</sub>, Met<sub>2</sub>, Ile<sub>4</sub>, Leu<sub>9</sub>, Tyr<sub>2</sub>, Phe<sub>3</sub>, Lys<sub>7</sub> and Arg<sub>1</sub>. The molecular weight of YPA1 is 11 020. The amino acid sequence was determined by <span><math><mtext>4-N,N-</mtext><mtext>dimethylaminoazobenzene</mtext></math></span> 4′-isothiocyanate degradation of the peptides obtained by digestions with trypsin, chymotrypsin, thermolysin, pepsin and <em>Staphylococcus aureus</em> protease of intact protein. A comparison of protein YPA1 from yeast with eL12 from <em>Artemia salina</em> shows a high sequence similarity. A considerable similarity is also shown with HL20 from <em>Halobacterium cutirubrum</em>. On the other hand, there is very little apparent sequence similarity between YPA1 and the eubacterial acidic protein L12 either from <em>E. coli</em> or <em>B. subtilis</em>.</p></div>","PeriodicalId":100165,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Protein Structure","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1981-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2795(81)90088-X","citationCount":"24","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Protein Structure","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/000527958190088X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 24

Abstract

The complete primary structure of an acidic ribosomal protein YPA1 from Saccharomyces cerevisiae has been determined. YPA1 is composed of 110 amino acid residues and has the composition: Asp7, Asn2, Thr2, Ser9, Glu15, Gln2, Pro3, Gly15, Ala21, Val6, Met2, Ile4, Leu9, Tyr2, Phe3, Lys7 and Arg1. The molecular weight of YPA1 is 11 020. The amino acid sequence was determined by 4-N,N-dimethylaminoazobenzene 4′-isothiocyanate degradation of the peptides obtained by digestions with trypsin, chymotrypsin, thermolysin, pepsin and Staphylococcus aureus protease of intact protein. A comparison of protein YPA1 from yeast with eL12 from Artemia salina shows a high sequence similarity. A considerable similarity is also shown with HL20 from Halobacterium cutirubrum. On the other hand, there is very little apparent sequence similarity between YPA1 and the eubacterial acidic protein L12 either from E. coli or B. subtilis.

酿酒酵母酸性核糖体蛋白YPA1的初级结构
已经确定了酿酒酵母酸性核糖体蛋白YPA1的完整一级结构。YPA1由110个氨基酸残基组成,其组成为:Asp7、Asn2、Thr2、Ser9、Glu15、Gln2、Pro3、Gly15、Ala21、Val6、Met2、Ile4、Leu9、Tyr2、Phe3、Lys7和Arg1。YPA1的分子量为11 020。用胰蛋白酶、凝乳胰蛋白酶、热溶酶、胃蛋白酶和金黄色葡萄球菌蛋白酶消化得到的肽段,用4- n, n -二甲氨基偶氮苯4′-异硫氰酸酯降解得到氨基酸序列。酵母中YPA1蛋白与盐渍蒿中eL12蛋白的序列相似性较高。与角质盐杆菌的HL20也有相当大的相似性。另一方面,无论是大肠杆菌还是枯草芽孢杆菌,YPA1与真菌性酸性蛋白L12之间的序列相似性都很小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信