Agreement and disagreement between laboratories in species identification of mycobacteria.

M Kubín, B Burjanová, A G Kchomenko, T B Iliina, M Janowiec, W Käppler, E Kubala, N B Makarewich, M Slosárek, M Vincúrová
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Abstract

Twenty coded strains of the following species: Mycobacterium avium, M. fortuitum, M. gordonae, M. chelonei, M. intracellulare, M. kansasii, M. nonchromogenicum, M. scrofulaceum, M. terrae, M. triviale and M. xenopi, were subjected to identification in a co-operative study undertaken by seven laboratories of four countries (CSSR, GDR, PRP and USSR). Three of these laboratories recognized 18-19 (90-95%) of the strains, three others 15-17 (75-85%) and one laboratory recognized 8 (40%) strains. In the correctly identified species, agreement between the tests used by all participants was evaluated. The highest rates of agreement in positive or negative results (04-100%) were obtained for nitrate reduction, detection of arylsulphatase, urease and nicotinamidase in M. kansasii, M. avium-intracellulare and M. fortuitum.

分枝杆菌种类鉴定实验室间的一致与分歧。
本文对禽分枝杆菌、福氏分枝杆菌、gordonae分枝杆菌、chelonei分枝杆菌、胞内分枝杆菌、kansasii分枝杆菌、nonchromogenicum分枝杆菌、scrofulaceum分枝杆菌、terrae分枝杆菌、triviale分枝杆菌和xenopi分枝杆菌等20个编码菌株进行了鉴定,由4个国家(CSSR、GDR、PRP和苏联)的7个实验室共同开展。其中3个实验室识别出18-19株(90-95%),3个实验室识别出15-17株(75-85%),1个实验室识别出8株(40%)。在正确识别的物种中,评估了所有参与者使用的测试之间的一致性。硝酸还原酶、芳基硫酸酶、脲酶和烟酰胺酶检测结果的符合率最高(04-100%),适用于甘肃分枝杆菌、鸟胞内分枝杆菌和福图菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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