{"title":"[Detection of hepatitis Be antigen with an enzyme immunoassay (author's transl)].","authors":"W Lange, K N Masihi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>An enzyme immunoassay (ELISA) from SEROMED was tested for its suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis. From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higherr than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somwhat lower sensitivity of ELISA. However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA. ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.</p>","PeriodicalId":23929,"journal":{"name":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 3","pages":"296-302"},"PeriodicalIF":0.0000,"publicationDate":"1980-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
An enzyme immunoassay (ELISA) from SEROMED was tested for its suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis. From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higherr than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somwhat lower sensitivity of ELISA. However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA. ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.
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