[Measurements of cell adhesion to biomaterials by supersonics (author's transl)].

Abstract

In order to examine eligibility of supersonic vibration energy for investigating cell-material contract, L-strain fibroblasts were cultivated on glass surface and exposed to supersonic vibration. The cells adhered to the glass surface were detached by supersonic vibration and both of adhered cells to the glass surface and detached cells after the supersonic exposure were cultivated for one week. And the cell activity of the detached cells was estimated with cell multiplication by cell nuclei counting and their morphological changes. As a result, use of supersonic vibration energy was found effective in expressing degrees of cell adhesiveness to glass or biomaterials. The most appropriate vibration was decided at 485 KHz in frequency, 5 V in secondary voltage and approximately 30 seconds. It is revealed that the results may be effective in the analysis of cell-biomaterial contact.