NAD+-dependent acetaldehyde oxidation in Drosophila

Progress in neuro-psychopharmacology Pub Date : 1981-01-01 DOI:10.1016/0364-7722(81)90059-X

Francoise Garcin, Denis Kasiencsuk, Simone Radouco-Thomas, Johanne Cote, Corneille Radouco-Thomas

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引用次数: 6

Abstract

1.
1. NAD⁺-dependent acetaldehyde oxidation was measured spectrophotometrically in homogenates of flies Drosophila melanogaster.
2.
2. The reaction was specifically triggered by addition of acetaldehyde and proceeded linearily over five minutes.
3.
3. At low acetaldehyde concentrations the reaction rate was rapidly maximal whereas higher acetaldehyde concentrations proved to be inhibitary.
4.
4. Enzyme activity in regard to NAD⁺ concentration followed classical Michaelis kinetics. The apparent Km for NAD⁺ was 0.05 mM.
5.
5. These data provide evidence for the presence of a NAD⁺-dependent aldehyde dehydrogenase. It is suggested that this enzyme is involved in the oxidation of acetaldehyde in Drosophila.
6.
6. The biochemical and biological significance of this enzyme in regard to ethanol metabolism and ethanol tolerance is discussed.

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果蝇NAD+依赖性乙醛氧化

1.1. 用分光光度法测定黑腹果蝇匀浆中NAD+依赖性乙醛氧化。该反应是由乙醛的加入引起的，并在5分钟内线性进行。在低乙醛浓度下，反应速率最快，而较高的乙醛浓度则被证明是抑制的。NAD+浓度对酶活性的影响遵循经典的米切里斯动力学。NAD+的视Km为0.05 mM.5.5。这些数据为NAD+依赖性醛脱氢酶的存在提供了证据。这表明这种酶参与果蝇乙醛的氧化。6.6。讨论了该酶在乙醇代谢和乙醇耐受性方面的生物化学和生物学意义。

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Progress in neuro-psychopharmacology

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