{"title":"NAD+-dependent acetaldehyde oxidation in Drosophila","authors":"Francoise Garcin, Denis Kasiencsuk, Simone Radouco-Thomas, Johanne Cote, Corneille Radouco-Thomas","doi":"10.1016/0364-7722(81)90059-X","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. NAD<sup>+</sup>-dependent acetaldehyde oxidation was measured spectrophotometrically in homogenates of flies Drosophila melanogaster.</p></span></li><li><span>2.</span><span><p>2. The reaction was specifically triggered by addition of acetaldehyde and proceeded linearily over five minutes.</p></span></li><li><span>3.</span><span><p>3. At low acetaldehyde concentrations the reaction rate was rapidly maximal whereas higher acetaldehyde concentrations proved to be inhibitary.</p></span></li><li><span>4.</span><span><p>4. Enzyme activity in regard to NAD<sup>+</sup> concentration followed classical Michaelis kinetics. The apparent Km for NAD<sup>+</sup> was 0.05 mM.</p></span></li><li><span>5.</span><span><p>5. These data provide evidence for the presence of a NAD<sup>+</sup>-dependent aldehyde dehydrogenase. It is suggested that this enzyme is involved in the oxidation of acetaldehyde in Drosophila.</p></span></li><li><span>6.</span><span><p>6. The biochemical and biological significance of this enzyme in regard to ethanol metabolism and ethanol tolerance is discussed.</p></span></li></ul></div>","PeriodicalId":20801,"journal":{"name":"Progress in neuro-psychopharmacology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0364-7722(81)90059-X","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Progress in neuro-psychopharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/036477228190059X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
1.
1. NAD+-dependent acetaldehyde oxidation was measured spectrophotometrically in homogenates of flies Drosophila melanogaster.
2.
2. The reaction was specifically triggered by addition of acetaldehyde and proceeded linearily over five minutes.
3.
3. At low acetaldehyde concentrations the reaction rate was rapidly maximal whereas higher acetaldehyde concentrations proved to be inhibitary.
4.
4. Enzyme activity in regard to NAD+ concentration followed classical Michaelis kinetics. The apparent Km for NAD+ was 0.05 mM.
5.
5. These data provide evidence for the presence of a NAD+-dependent aldehyde dehydrogenase. It is suggested that this enzyme is involved in the oxidation of acetaldehyde in Drosophila.
6.
6. The biochemical and biological significance of this enzyme in regard to ethanol metabolism and ethanol tolerance is discussed.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
