Thelma C. Fletcher , Ann White , Arthur Youngson , Arpad Pusztai , Brian A. Baldo
{"title":"Characterization of C-reactive protein from the eggs of the marine teleost, cyclopterus lumpus L.","authors":"Thelma C. Fletcher , Ann White , Arthur Youngson , Arpad Pusztai , Brian A. Baldo","doi":"10.1016/0005-2795(81)90092-1","DOIUrl":null,"url":null,"abstract":"<div><p>Further evidence is presented for the existence in teleost fish of proteins homologous with mammalian C-reactive protein. The amino acid composition is given for a C-reactive protein isolated from the eggs of a marine teleost, <em>Cyclopterus lumpus</em>, by extraction with lecithin in the presence of Ca<sup>2+</sup>, followed by electrofocusing. A molecular weight of 150000 was calculated from gel filtration and electrophoresis at different polyacrylamide gel concentrations, while the <span><math><mtext>s</mtext><msub><mi></mi><mn><mtext>20,</mtext><mtext>w</mtext></mn></msub></math></span> was 7.4 S. The 1.5-S subunits had an apparent <span><math><mtext>M</mtext><msub><mi></mi><mn><mtext>r</mtext></mn></msub></math></span> of 20 000 by SDS-polyacrylamide gel electrophoresis and 21 000 by computer analysis based on amino acid composition. Comparison is made with the physicochemical properties of mammalian C-reactive protein.</p></div>","PeriodicalId":100165,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Protein Structure","volume":"671 1","pages":"Pages 44-49"},"PeriodicalIF":0.0000,"publicationDate":"1981-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2795(81)90092-1","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Protein Structure","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005279581900921","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
Further evidence is presented for the existence in teleost fish of proteins homologous with mammalian C-reactive protein. The amino acid composition is given for a C-reactive protein isolated from the eggs of a marine teleost, Cyclopterus lumpus, by extraction with lecithin in the presence of Ca2+, followed by electrofocusing. A molecular weight of 150000 was calculated from gel filtration and electrophoresis at different polyacrylamide gel concentrations, while the was 7.4 S. The 1.5-S subunits had an apparent of 20 000 by SDS-polyacrylamide gel electrophoresis and 21 000 by computer analysis based on amino acid composition. Comparison is made with the physicochemical properties of mammalian C-reactive protein.