Characterization of C-reactive protein from the eggs of the marine teleost, cyclopterus lumpus L.

Thelma C. Fletcher , Ann White , Arthur Youngson , Arpad Pusztai , Brian A. Baldo
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引用次数: 10

Abstract

Further evidence is presented for the existence in teleost fish of proteins homologous with mammalian C-reactive protein. The amino acid composition is given for a C-reactive protein isolated from the eggs of a marine teleost, Cyclopterus lumpus, by extraction with lecithin in the presence of Ca2+, followed by electrofocusing. A molecular weight of 150000 was calculated from gel filtration and electrophoresis at different polyacrylamide gel concentrations, while the s20,w was 7.4 S. The 1.5-S subunits had an apparent Mr of 20 000 by SDS-polyacrylamide gel electrophoresis and 21 000 by computer analysis based on amino acid composition. Comparison is made with the physicochemical properties of mammalian C-reactive protein.

海洋硬骨鱼(cyclopterus lumpus L.)卵中c反应蛋白的表征。
进一步的证据表明,硬骨鱼中存在与哺乳动物c反应蛋白同源的蛋白质。氨基酸组成给出了从海洋硬骨鱼(Cyclopterus lumpus)的卵中分离出的c反应蛋白,通过在Ca2+存在下用卵磷脂提取,然后电聚焦。在不同浓度的聚丙烯酰胺凝胶下,通过凝胶过滤和电泳计算得到分子量为150000,其中s20w为7.4 s, sds -聚丙烯酰胺凝胶电泳的表观Mr为20000,基于氨基酸组成的计算机分析的表观Mr为21000。并与哺乳动物c反应蛋白的理化性质进行了比较。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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