Preparation of specific Escherichia coli K88 antisera by means of purified K88ab and K88ad antigens.

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1980-11-01

P A Guinée, F R Mooi, W H Jansen

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Abstract

Escherichia coli K88ab and K88ad antigens were isolated from K88 antigen-producing E. coli K12 transconjugants and purified by means of repeated column chromatography. Injection of about 1 to 2 mg of the purified antigens into rabbits yielded specific K88ab and K88ad antisera. Attempts to obtain specific K88ac antiserum by means of this procedure failed.

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纯化K88ab和K88ad抗原制备特异性大肠杆菌K88抗血清。

从产K88抗原的大肠杆菌K12转偶联物中分离到大肠杆菌K88ab和K88ad抗原，采用重复柱层析法进行纯化。将纯化后的抗原约1 ~ 2mg注射到家兔体内，产生特异性的K88ab和K88ad抗血清。试图通过这种方法获得特异性K88ac抗血清失败。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie

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