[Melanotropic hormone of goldfish (Carassius auratus L.): radioimmunologic comparison with synthetic alpha-MSH].

Abstract

alpha MSH from the neuro-intermediate lobe of the pituitary gland of the goldfish displays a competitive behaviour in an RIA for synthetic alpha MSH. The inhibition curves from neuro-intermediate homogenates parallel the standard curve (P < 0,01) but two major pools of immunoreactive peptides have been separated by Sephadex gel chromatography. The neuro-intermediate lobe contains 480 +/- 56 ng (S.E.M.) of immunoreactive alpha MSH.