Helper cells from lymph node or spleen induce different 7S antibody responses.

Abstract

The helper activity of GAT (L-glutamic acid 60-L-alanine 30-L-tyrosine 10)-specific T cells from BALB/c mice has been characterized. The isotypic pattern of the PFC response (plaque-forming cell) obtained in vitro is different depending on the origin of the T helper cells. T helper cells from primed spleen induce a very predominant IgG1 PFC response while T helper cells from primed lymph node induce IgG1-IgG2a- and IgG2b-PFC responses. When primed spleen cells are added to primed lymph node cells in vitro, the IgG2a- and IgG2b-PFC responses are abolished. This results suggests the presence of isotype-specific suppressor cells in the spleen of immunized mice.