Anamnestic chemiluminescence of murine spleen cells.

L S Wong, J L Kiel
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引用次数: 1

Abstract

The oxidative response of murine spleen cells to secondary exposure to antigen was determined by luminol (5-amino-2,3-dihydro-1,4-pthalazinedione) amplified chemiluminescence, CL. BALB/cj and CBA/J mice were immunized with saline or an antigen solution of saline, luminol, and bovine serum albumin. Spleen cells were obtained from mice two and four days after immunization, and the CL response to in vitro antigenic exposure was measured for 35 minutes. At two days post-immunization, there was no difference in the CL of control and antigen-primed cells. By day four, the antigen-primed CL response differed significantly in both magnitude and time course from the primary antigen-stimulated response of the controls. This early development of differential CL response to antigenic challenge suggests a role for oxidative metabolic activity in the expression of the anamnestic immune response.

小鼠脾细胞的遗忘性化学发光。
采用鲁米诺(5-氨基-2,3-二氢-1,4-酞嗪二酮)扩增化学发光法测定小鼠脾细胞对抗原二次暴露的氧化反应。用生理盐水或生理盐水、鲁米诺和牛血清白蛋白组成的抗原溶液免疫BALB/cj和CBA/J小鼠。免疫后2天和4天分别取小鼠脾细胞,测定体外抗原暴露35分钟后的CL反应。免疫后2天,对照和抗原引物细胞的CL无差异。到第四天,抗原引发的CL反应在强度和时间上与对照组的主要抗原刺激反应有显著差异。这种对抗原攻击的差异CL反应的早期发展表明氧化代谢活性在遗忘免疫反应的表达中起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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