Effect of clonal senescence on low density lipoprotein-receptor activity of bovine arterial endothelial cells.

Abstract

Senescent and young bovine arterial endothelial cells derived from the same parental cell clone were compared to test the effect of in vitro endothelial cell senescence on low density lipoprotein (LDL) and modified LDL-receptor activities. Low density lipoprotein binding and degradation were both increased in cells that underwent a larger number of population doublings, whereas acetyl LDL binding and degradation were unchanged. The increased LDL-receptor activity associated with endothelial cell senescence remained significant after variation of cell number among senescent and young clones was taken into account. Thus, aging endothelial cells seem capable of continuing to process LDL and modified LDL, which could play a role in the arterial wall changes that occur with age in vivo.