Optimization of the culture medium composition for the antibody response of mouse spleen cells.

In Vitro Pub Date : 1984-08-01 DOI:10.1007/BF02619610

D Zhu, I Lefkovits

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引用次数: 3

Abstract

The Mishell-Dutton culture system for in vitro primary antibody response of mouse spleen cells was used to optimize the amino acid composition of RPMI 1640 media. Each of the 20 amino acids was tested over a broad range of concentrations always leaving the remaining 19 amino acids unaltered (i.e. at the formula recommended concentration). In several instances, higher plaque-forming cell responses were obtained with an amino acid concentration that was either higher or lower than that recommended: (a) the optimum concentration for valine, glutamine, and lysine lies considerably above the recommended one, (b) the optimum concentration for leucine as well as for several other amino acids lies below the recommended concentration, and (c) the optimum concentration for arginine corresponds exactly to the recommended concentration. The second round of optimization, i.e. combining of two conditions that individually yielded an improved response often caused a decrease of response. The possibility is discussed that for an optimal response a ratio of two or several amino acids rather than the absolute concentration of any one amino acid is of importance.

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小鼠脾细胞抗体应答培养基组成的优化。

采用小鼠脾细胞体外一抗反应的michell - dutton培养系统对RPMI 1640培养基的氨基酸组成进行优化。20种氨基酸中的每一种都在广泛的浓度范围内进行测试，始终保持其余19种氨基酸不变(即在配方推荐浓度下)。在一些情况下，当氨基酸浓度高于或低于推荐浓度时，会获得更高的斑块形成细胞反应:(a)缬氨酸、谷氨酰胺和赖氨酸的最佳浓度大大高于推荐浓度，(b)亮氨酸以及其他几种氨基酸的最佳浓度低于推荐浓度，(c)精氨酸的最佳浓度与推荐浓度完全一致。第二轮优化，即结合两个单独产生改进响应的条件，通常会导致响应减少。讨论了两种或几种氨基酸的比例比任何一种氨基酸的绝对浓度更重要，以获得最佳反应的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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In Vitro

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