{"title":"Polymer cellulose sheets immobilizing antibody by radiation polymerization.","authors":"M Kumakura, I Kaetsu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Antibody such as anti-alpha-fetoprotein was immobilized on cellulose fibril sheets by radiation polymerization of hydrophilic monomers. The nature of the immobilized anti-alpha-fetoprotein sheets was examined by measuring the enzyme activity in an antibody-antigen complex reaction. The relationship between the activity and the polymerization condition was studied. The degree of hydration of the polymer matrix giving a maximum activity appeared to be about 0.2. The activity varied with monomer concentration and with monomer composition in copolymerization of acrylate and diacrylate monomer. The activity was increased by the addition of polyethylene glycol diacrylate monomers, in which the activity increased with increasing length of the oxyethylene unit chain.</p>","PeriodicalId":14978,"journal":{"name":"Journal of applied biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1983-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of applied biochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Antibody such as anti-alpha-fetoprotein was immobilized on cellulose fibril sheets by radiation polymerization of hydrophilic monomers. The nature of the immobilized anti-alpha-fetoprotein sheets was examined by measuring the enzyme activity in an antibody-antigen complex reaction. The relationship between the activity and the polymerization condition was studied. The degree of hydration of the polymer matrix giving a maximum activity appeared to be about 0.2. The activity varied with monomer concentration and with monomer composition in copolymerization of acrylate and diacrylate monomer. The activity was increased by the addition of polyethylene glycol diacrylate monomers, in which the activity increased with increasing length of the oxyethylene unit chain.
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