Characterization of the autoimmune antigenic determinant for ribonucleoprotein (RNP) antibody.

P F Agris, Y KiKuchi, H J Gross, M Takano, G C Sharp
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引用次数: 14

Abstract

Small nuclear ribonucleoprotein complexes are antigens in various autoimmune diseases. The serological pattern of high titers of circulating antibody to nuclear ribonucleoprotein (RNP) antigen is a diagnostic marker for mixed connective tissue disease (MCTD); whereas antibody to Sm is prevalent in systemic lupus erythematosus (SLE). Both calf thymus and rabbit thymus are commonly used, excellent sources for preparation of the corresponding antigens RNP and Sm in clinical and research laboratories (A. M. Boak et al., accompanying paper). Thus, biochemical and structural characterization of the minimal antigenic determinant in these preparations is important for its use in the laboratory, as well as significant for understanding MCTD, SLE, and other examples of autoimmunity. Purification and biochemical analyses of immunologically active RNP from many different preparations of calf thymus extract has revealed that the majority of antibody in monospecific MCTD patient sera recognizes an antigen composed of the 165 nucleotide RNA, U1 RNA, and five peptides. Calf thymus U1 RNA was found to be identical in sequence to that of man. A sequence of 55 nucleotides within the 165 nucleotide RNA was the minimal RNA fragment found in RNP particles that were still immunologically active. Two of the RNP peptides react with patient sera monospecific for RNP and thus, are presumably the antigenic peptides complexed with the 55 nucleotide RNA sequence.

核糖核蛋白(RNP)抗体自身免疫抗原决定因素的表征。
小核核糖-核蛋白复合物是多种自身免疫性疾病的抗原。高滴度循环核核糖核蛋白(RNP)抗原抗体的血清学模式是混合性结缔组织病(MCTD)的诊断标志;而Sm抗体在系统性红斑狼疮(SLE)中普遍存在。小牛胸腺和兔胸腺都是常用的,是临床和研究实验室制备相应抗原RNP和Sm的绝佳来源(A. M. Boak等,随附论文)。因此,这些制剂中最小抗原决定因子的生化和结构特征对于其在实验室中的应用非常重要,对于理解MCTD、SLE和其他自身免疫的例子也很重要。从许多不同制备的小牛胸腺提取物中提取的免疫活性RNP的纯化和生化分析表明,单特异性MCTD患者血清中的大多数抗体识别由165个核苷酸RNA, U1 RNA和5个肽组成的抗原。小牛胸腺U1 RNA的序列与人类相同。165个核苷酸RNA中的55个核苷酸序列是在RNP颗粒中发现的仍具有免疫活性的最小RNA片段。两种RNP肽与患者血清单特异性RNP反应,因此,可能是抗原肽与55核苷酸RNA序列复合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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